Identification of the promoter region and genetic mutations of the porcine GALP gene
Identification of the promoter region and genetic mutations of the porcine GALP gene
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DOI：
10.1007/s11033-012-2297-1
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发表时间：
2013-04
影响因子：
2.8
通讯作者：
Lina Su;S. Mei;H. Tao;Xianwen Peng;Xiaojie Sun;Huayu Wu;Xuying Zhang;M. Qiao;Fenge Li
中科院分区：
文献类型：
--
作者：
Lina Su;S. Mei;H. Tao;Xianwen Peng;Xiaojie Sun;Huayu Wu;Xuying Zhang;M. Qiao;Fenge Li
GALP （galanin -like peptide， GALP）基因是一种与生殖有关的神经肽，在PMSG-hCG刺激的中国太湖母猪和大白母猪排卵前卵泡中存在差异表达。本研究确定了猪egalpgene的启动子区域和基因突变。利用神经网络启动子预测2.2版本预测了一个位于5 '侧区1322 bp的序列包含5个潜在的转录启动子。CHO细胞和hela细胞中5′-缺失的表达表明，在- 852 ~ - 803 bp处有一个负调控元件，在- 1318 ~ - 1269 bp处有一个正调控元件。对中国太湖和大白垩基因序列的比较分析揭示了这一现象。* 3 ' -UTR中的27C>突变。内含子1中88-1225C >突变，分别可以通过hhai和alui PCR-RFLP检测到。与产仔数性状的关联分析显示，各胎次DIV猪的NBA在CC位点和GG基因型上存在差异（P< 0.05）。但是，用SHEsis在线软件分析，两个snp不存在显著的连锁不平衡，可以单独用于猪的育种。
Galanin-like peptide (GALP) gene, encoding a member of the galanin family of neuropeptides involved in reproduction, was differentially expressed in PMSG-hCG stimulated pre-ovulatory ovarian follicles of Chinese Taihu and Large White sows in our previous study. In the present study, promoter region and genetic mutations of the porcineGALPgene were determined. A 1,322 bp contig in 5′-flanking region was predicted to contain 5 potential transcription promoters by Neural Network Promoter Prediction version 2.2. 5′-deletion expression in both CHO and hela cells showed that there were a negative regulatory element at −852 to −803 bp and a positive regulatory element at −1,318 to −1,269 bp. Comparative sequence analyses of Chinese Taihu and Large WhiteGALPgene sequence revealed thec.*27C>Gmutation in the 3′-UTR and thec.88–1225C>Gmutation in intron 1, which can be detected byHhaI andAluI PCR–RFLP, respectively. The association analysis with litter size traits showed that at both loci CC and GG genotypes were different for NBA for all parities in DIV pigs (P< 0.05). However, two SNPs were not in significant linkage disequilibrium analyzed using SHEsis online software, and could be used in pig breeding individually.