Assessment of vitamin A status by the deuterated-retinol-dilution technique and comparison with hepatic vitamin A concentration in Bangladeshi surgical patients
Assessment of vitamin A status by the deuterated-retinol-dilution technique and comparison with hepatic vitamin A concentration in Bangladeshi surgical patients
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DOI：
10.1093/ajcn/66.1.67
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发表时间：
1997-07-01
影响因子：
7.1
通讯作者：
Brown, KH
中科院分区：
文献类型：
--
作者：
Haskell, MJ;Handelman, GJ;Brown, KH
通过氘代视黄醇稀释（DRD）技术和分析在先前预定的腹部手术期间获得的肝活检标本的维生素A浓度，估计31名孟加拉国外科患者（15名男性和16名女性）的维生素A的肝脏储存。患者年龄范围为21 - 65岁，平均体重指数（BMI;单位：kg/m2）为17.7 +/- 3.4，术前9-11天口服0.753 μ mol [H-2（4）]醋酸视黄酯/kg体重。根据Fun et al（Am J Clin Nutr 1989;49：713-6），使用单次血浆同位素比测量（给药后18-25 d）估计肝脏维生素A储备。两种技术估计的平均肝脏维生素A储备相似，0.110 +/- 0.072 mmol（DRD）与0.100 +/- 0.067 mmol（活检）。回归分析用于比较DRD和活检技术的结果。两种技术之间存在显著的线性关系（r = 0.75，P < 0.0001），最小二乘回归直线y = x无显著性差异（P = 0.09）。结果表明，DRD技术提供了一个非常好的估计肝脏维生素A储备为这一人群。然而，观察到个体受试者肝脏维生素A储备估计值的预测区间较宽。因此，进一步完善的预测模型是必要的，以提高估计肝脏维生素A储备的个别受试者。
Hepatic stores of vitamin A were estimated in 31 Bangladeshi surgical patients (15 males and 16 females) by the deuterated-retinol-dilution (DRD) technique and by analysis of the vitamin A concentration of a liver biopsy specimen obtained during previously scheduled abdominal surgery. Patients ranged in age from 21 to 65 y and had an average body mass index (BMI; in kg/m(2)) Of 17.7 +/- 3.4 They received 0.753 mu mol [H-2(4)]retinyl acetate/kg body wt orally 9-11 d before surgery. Hepatic vitamin A reserves were estimated according to Fun et al (Am J Clin Nutr 1989;49:713-6) by using a single plasma isotopic-ratio measurement (18-25 d postdose). Estimated mean hepatic vitamin A stores were similar by both techniques, 0.110 +/- 0.072 mmol (by DRD) compared with 0.100 +/- 0.067 mmol (by biopsy). Regression analysis was used to compare results of the DRD and biopsy techniques. A significant linear relation was found between the two techniques (r = 0.75, P < 0.0001), and the least-squares regression line was not significantly different from y = x (P = 0.09). The results indicate that the DRD technique provided a very good estimate of hepatic vitamin A reserves for this population. However, a wide prediction interval was observed for estimates of hepatic vitamin A reserves for individual subjects. Thus, further refinement of the prediction model is necessary to improve estimates of hepatic vitamin A reserves for individual subjects.