Genetic effect of anatase on osteoblast-like cells.
Genetic effect of anatase on osteoblast-like cells.
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锐钛矿对成骨细胞样细胞的遗传效应。
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
F. Carinci
中科院分区：
文献类型：
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作者：
V. Sollazzo;A. Palmieri;F. Pezzetti;A. Scarano;M. Martinelli;L. Scapoli;L. Massari;G. Brunelli;E. Caramelli;F. Carinci
钛具有良好的机械和化学性能，是假体材料中的黄金标准。当暴露在氧气中时，钛成为一种生物相容的氧化物，能够诱导骨整合。二氧化钛有三种同素异形体，即钛铁矿、金红石和锐钛矿。锐钛矿可以制备成胶体悬浮液，然后用于表面涂层。锐钛矿涂层(AC)可能具有特定的生物效应。在这里，我们正在测试AC对成骨样细胞(MG63)的影响，方法是使用微阵列技术来识别暴露于AC的成骨细胞中不同调控的基因。通过包含20,000个基因的DNA芯片，我们在AC上培养的成骨样细胞系(MG-63)中鉴定了几个表达显著上调或下调的基因。它们涵盖了广泛的功能活动：信号转导、免疫、细胞周期调节、溶酶体组成和囊泡运输、细胞黏附、细胞骨架和细胞外基质成分、增殖和凋亡。据我们所知，所报道的数据构成了AC效应的第一个基因画像。它们可以更好地理解骨再生的分子机制，并可以作为比较其他具有类似临床效果的材料的模型。
Titanium is the gold standard among materials used for prosthetic devices, because of its good mechanical and chemical properties. When exposed to oxygen, titanium becomes an oxide that is biocompatible and able to induce osseointegration. Three allotropic forms of titanium dioxide exist, that is brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and then used to coat surfaces. Anatase coating (AC) can potentially have specific biological effects. Here we are testing the effect of AC on osteoblast-like cells (MG63) by using microarray techniques to identify genes that are differently regulated in osteoblasts exposed to AC. By using DNA microarrays containing 20,000 genes, we identified in osteoblast-like cell lines (MG-63) cultured on AC, several genes whose expression was significantly up- or downregulated. They cover a broad range of functional activities: signaling transduction, immunity, cell cycle regulation, lysosomes composition and vesicular transport, cell adhesion, cytoskeleton and extracellular matrix components, proliferation, and apoptosis. The data reported constitute, to our knowledge, the first genetic portrait of AC effects. They can be relevant to a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.
DOI：
10.1073/pnas.91.7.2752
发表时间：
1994-03-29
影响因子：
11.1
作者：
CHARO, IF;MYERS, SJ;COUGHLIN, SR
通讯作者：
COUGHLIN, SR