Storage of human plasma samples leads to alterations in the lipoprotein distribution of apoC-III and apoE
Storage of human plasma samples leads to alterations in the lipoprotein distribution of apoC-III and apoE
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DOI：
10.1194/jlr.d300041-jlr200
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发表时间：
2004-08-01
影响因子：
6.5
通讯作者：
Davignon, A
中科院分区：
文献类型：
--
作者：
Cohn, JS;Rodriguez, C;Davignon, A
采用酶联免疫吸附法（ELISA）测定16例冻存2周前后血浆中HDL和载脂蛋白B（apoB）的载脂蛋白C-Ⅲ（apoC-Ⅲ）和载脂蛋白E（apoE）的含量，研究冻存对载脂蛋白C-Ⅲ（apoC-Ⅲ）和载脂蛋白E（apoE）分布的影响。通过肝素锰沉淀（HMP）或快速蛋白液相色谱（FPLC）分离HDL。总血浆apoC-III和apoE水平不受冷冻储存的影响。冷冻样本中的HDL-HMP apoC-III和apoE水平显著高于新鲜样本：分别为7.7 +/-0.7 vs 6.7 +/- 0.7 mg/dl（P < 0.05）和2.0 +/- 0.1 vs 1.2 +/- 0.1 mg/dl（P < 0.001）。HDL-FPLC apoC-III和apoE水平在冷冻样本中也更高，但甘油三酯（TG）或胆固醇水平不高：分别为2.0 +/- 1.2 vs 7.5 +/- 0.6 mg/dl（P < 0.001）和2.7 +/- 0.2 vs 1.6 +/- 0.2 mg/dl（P < 0.001）。冷冻储存导致富含甘油三酯脂蛋白中apoC-III（-17 +/-9%）和apoE（-19 +/-9%）减少。apoC-III和apoE的再分布在高TG水平的样本中最为明显。当在-80 ℃下储存的血浆中测量时，HDL apoC-III和apoE水平也显著更高。我们的结果表明，apoC-III和apoE的脂蛋白分布受人血浆储存的影响，这表明在将apoC-III和/或apoE的脂蛋白水平与冠状动脉疾病发病率相关的研究中应避免分析冷冻血浆。科恩，J.S.，C.罗德里格斯，H。Jacques，M. Tremblay，and J. Davignon.人血浆样品的储存导致apoC-III和apoE的脂蛋白分布的改变。
The effect of frozen storage on lipoprotein distribution of apolipoprotein C-III (apoC-III) and apoE was investigated by measuring apoC-III and apoE by ELISA in HDL and apoB-containing lipoproteins of human plasma samples (n = 16) before and after 2 weeks of frozen storage (-20degreesC). HDLs were separated by heparin-manganese precipitation (HMP) or by fast-protein liquid chromatography (FPLC). Total plasma apoC-III and apoE levels were not affected by frozen storage. HDL-HMP apoC-III and apoE levels were significantly higher in frozen versus fresh samples: 7.7 +/- 0.7 versus 6.7 +/- 0.7 mg/dl (P < 0.05) and 2.0 +/- 0.1 versus 1.2 +/- 0.1 mg/dl (P < 0.001), respectively. HDL-FPLC apoC-III and apoE, but not triglyceride (TG) or cholesterol, levels were also higher in frozen samples: 2.0 +/- 1.2 versus 7.5 +/- 0.6 mg/dl (P < 0.001) and 2.7 +/- 0.2 versus 1.6 +/- 0.2 mg/dl (P < 0.001), respectively. Frozen storage led to a decrease in apoC-III (-17 +/- 9%) and apoE (-19 +/- 9%) in triglyceride-rich lipoprotein. Redistribution of apoC-III and apoE was most evident in samples with high TG levels. HDL apoC-III and apoE levels were also significantkly higher when measured in plasma stored at - 80degreesC.square Our results demonstrate that lipoprotein distribution of apoC-III and apoE is affected by storage of human plasma, suggesting that analysis of frozen plasma should be avoided in studies relating lipoprotein levels of apoC-III and/or apoE to the incidence of coronary artery disease.-Cohn, J. S., C. Rodriguez, H. Jacques, M. Tremblay, and J. Davignon. Storage of human plasma samples leads to alterations in the lipoprotein distribution of apoC-III and apoE.