Quantitative differences in intestinal Faecalibacterium prausnitzii in obese Indian children
Quantitative differences in intestinal Faecalibacterium prausnitzii in obese Indian children
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DOI：
10.1017/s0007114509992182
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发表时间：
2010-02-14
影响因子：
3.6
通讯作者：
Ramakrishna, Balakrishnan S.
中科院分区：
文献类型：
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作者：
Balamurugan, Ramadass;George, Gemlyn;Ramakrishna, Balakrishnan S.
肠道细菌通过发酵未吸收的碳水化合物的能力来帮助人类保存能量。本研究检查了肥胖和非肥胖儿童的主要粪便微生物群的组成。年龄在11-14岁的参与者（n 28）提供新鲜粪便样本，并完成了饮食调查，包括24小时饮食回忆和前3个月常用食物的FFQ。使用靶向16 S rDNA的引物通过实时PCR定量粪便细菌。在参与者中，15名（7名女性）肥胖，年龄组BMI中位数为第99百分位数（范围97至> 99），而13名参与者（7名女性）体重正常，年龄组BMI中位数为第50百分位数（范围1-85）。肥胖和非肥胖参与者之间的能量、碳水化合物、脂肪和蛋白质消耗没有显著差异。两组粪便中类杆菌-普雷沃氏菌水平无显著差异。双歧杆菌属、嗜酸乳杆菌群或直肠真杆菌。肥胖儿童的普氏粪杆菌水平显著高于非肥胖儿童（P=0.0253）。我们的结论是，发现增加的数字关闭。印度南部肥胖儿童粪便中的prausnitzii增加了肥胖症粪便微生物群改变的信息。
Gut bacteria contribute to energy conservation in man through their ability to ferment Unabsorbed carbohydrate. The present study examined the composition of predominant faecal microbiota in obese and non-obese children. The participants (n 28) aged 11-14 years provided fresh faecal samples and completed a dietary survey consisting of 24 h diet recall and a FFQ of commonly used foods taken over the previous 3 months. Faecal bacteria were quantitated by real-time PCR using primers targeted at 16S rDNA. Of the participants, fifteen (seven female) were obese, with median BMI-for-age at the 99th percentile (range 97 to > 99) while thirteen participants (seven female) were normal weight, with median BMI-for age being at the 50th percentile (range 1-85). Consumption of energy, carbohydrates, fat and protein was not significantly different between the obese and non-obese participants. There was no significant difference between the two groups in faecal levels of Bacteroides-Prevotella. Bifidobacterium species, Lactobacillus acidophilus group or Eubacterium rectale. Levels of Faecalibacterium prausnitzii, were significantly higher in obese children than in non-obese participants (P=0.0253). We concluded that the finding of increased numbers off. prausnitzii in the faeces of obese children in south India adds to the growing information on alterations in faecal microbiota in obesity.