Combination of hydrothermal pretreatment and sodium hydroxide post-treatment applied on wheat straw for enhancing its enzymatic hydrolysis
Combination of hydrothermal pretreatment and sodium hydroxide post-treatment applied on wheat straw for enhancing its enzymatic hydrolysis
复制标题
水热预处理与氢氧化钠后处理相结合增强麦秆酶解作用
DOI：
10.1007/s10570-017-1644-8
复制
发表时间：
2018-02-01
期刊：
影响因子：
5.7
通讯作者：
Wang, Shuangfei
中科院分区：
文献类型：
--
作者：
Min, Douyong;Wei, Lei;Wang, Shuangfei
分别用水、稀硫酸和氢氧化钠溶液对小麦秸秆进行高温预处理。在预处理中，水热处理进行了不同的严重程度。水热预处理样品和稀酸预处理样品分别在室温下用氢氧化钠溶液萃取后处理。然后对预处理的样品进行酶解以评估还原糖回收率。结果表明，碱预处理能有效去除木质素。而水热预处理和稀酸预处理使大量的半纤维素溶解。水热预处理程度影响半纤维素的溶解。碱预处理的样品实现了最高的还原糖回收率，例如38.5g糖/100 g小麦秸秆。碱性后处理通过去除额外的半纤维素和木质素来增强酸性预处理样品的酶水解。还原糖得率最高为43.6 g/100 g麦草，表明水热预处理与碱提相结合是一种很有前途的还原糖生产方法。
Wheat straw was respectively pretreated by water, dilute sulfuric acid, and sodium hydroxide solution at elevated temperature. Among the pretreatments, hydrothermal process was carried out with different severities. Hydrothermal pretreated sample and dilute acid pretreated sample were subsequently extracted by sodium hydroxide solution at room temperature which was applied as post-treatment. The pretreated samples were then enzymatically hydrolyzed to evaluate reducing sugar recovery. The result indicated that alkaline pretreatment removed a significant amount of lignin. While hydrothermal pretreatment and dilute acid pretreatment solubilized a significant amount of hemicellulose. And the hydrothermal pretreatment severity affected the solubilization of hemicellulose. The alkaline pretreated sample achieved the highest reducing sugar recovery e.g. 38.5 g sugar/100 g wheat straw. The alkaline post-treatment enhanced the enzymatic hydrolysis of acidic pretreated samples by removing additional hemicellulose and lignin. The best reducing sugar recovery was obtained at 43.6 g sugar/100 g wheat straw which indicated that the combination of hydrothermal pretreatment and alkaline extraction is a promising method in terms of reducing sugar production.