SPATIAL SEGREGATION OF ODORANT RECEPTOR EXPRESSION IN THE MAMMALIAN OLFACTORY EPITHELIUM
SPATIAL SEGREGATION OF ODORANT RECEPTOR EXPRESSION IN THE MAMMALIAN OLFACTORY EPITHELIUM
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DOI：
10.1016/0092-8674(93)90422-m
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发表时间：
1993-07-30
期刊：
影响因子：
64.5
通讯作者：
AXEL, R
中科院分区：
文献类型：
--
作者：
VASSAR, R;NGAI, J;AXEL, R
气味配体与嗅觉感觉神经元上的受体相互作用所引发的信号必须由大脑解码，以确定众多受体中哪些被激活了。我们已经研究了大鼠嗅上皮中气味受体表达的模式，以确定哺乳动物嗅觉系统是否利用感觉输入的空间分隔来对气味刺激的特性进行编码。用11种不同气味受体的探针进行的原位杂交实验表明，表达不同受体的感觉神经元在拓扑学上被分隔到嗅上皮内少数几个广泛但有界限的区域。然而，在给定的区域内，表达特定受体的嗅觉神经元似乎是随机分布的，而不是空间定位的。因此，复杂的哺乳动物嗅觉系统可能将上皮分隔成解剖学和功能上离散的单元，使得每个区域仅表达整个受体库的一个子集。
The signal elicited by the interaction of odorous ligands with receptors on olfactory sensory neurons must be decoded by the brain to determine which of the numerous receptors have been activated. We have examined the-patterns of odorant receptor expression in the rat olfactory epithelium to determine whether the mammalian olfactory system employs spatial segregation of sensory input to encode the identity of an odorant stimulus. In situ hybridization experiments with probes for 11 different odorant receptors demonstrate that sensory neurons expressing distinct receptors are topologically segregated into a small number of broad, yet circumscribed, zones within the olfactory epithelium. Within a given zone, however, olfactory neurons expressing a specific receptor appear to be randomly distributed, rather than spatially localized. The complex mammalian olfactory system may therefore compartmentalize the epithelium into anatomically and functionally discrete units, such that each zone expresses only a subset of the entire receptor repertoire.