Nectin‐1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb
Nectin‐1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb
复制标题
DOI：
10.1002/cne.23762
复制
发表时间：
2015-08
影响因子：
2.5
通讯作者：
Takahito Inoue;Takeshi Fujiwara;Y. Rikitake;T. Maruo;K. Mandai;K. Kimura;T. Kayahara;Shujie Wang;Yu Itoh;Kousyoku Sai;M. Mori;K. Mori;A. Mizoguchi;Y. Takai
中科院分区：
文献类型：
--
作者：
Takahito Inoue;Takeshi Fujiwara;Y. Rikitake;T. Maruo;K. Mandai;K. Kimura;T. Kayahara;Shujie Wang;Yu Itoh;Kousyoku Sai;M. Mori;K. Mori;A. Mizoguchi;Y. Takai
在嗅球的外丛状层（EPL）中，二尖瓣细胞伸出侧树突，其接触其他二尖瓣细胞的侧树突和初级树突以及颗粒细胞树突。这些树突状结构对于气味信息处理至关重要，但它们是如何形成的仍然未知。在免疫荧光显微镜下，细胞粘附分子nectin-1的免疫荧光信号集中在发育中小鼠嗅球EPL的二尖瓣细胞侧树突上。在电子显微镜下，nectin-1的免疫金颗粒对称地定位于二尖瓣细胞侧树突之间接触处的质膜上，其显示双侧变暗，没有致密的细胞骨架底涂层，这是粘附点连接的特征。我们将nectin-1的免疫金颗粒对称聚集的接触点命名为“nectin-1点”。nectin-1斑点的平均长度为0.21 μm，质膜之间的距离平均为20.8 nm。在连续切片的3D重建中，nectin-1斑点的簇形成了盘状结构。在nectin-1敲除小鼠的二尖瓣细胞侧树突中，未检测到nectin-1的免疫金颗粒，质膜变暗在电子显微镜下正常化，但质膜部分相互分离。在二尖瓣细胞侧树突和初级树突之间以及二尖瓣细胞侧树突和颗粒细胞树突棘颈之间进一步鉴定了nectin-1点。这些结果表明，nectin-1点构成了一种新的粘附装置，将二尖瓣细胞树突束缚在发育中小鼠嗅球的树突状网状结构中。J. Comp.神经元523：1824-1839，2015.© 2015 Wiley Periodicals，Inc.
Mitral cells project lateral dendrites that contact the lateral and primary dendrites of other mitral cells and granule cell dendrites in the external plexiform layer (EPL) of the olfactory bulb. These dendritic structures are critical for odor information processing, but it remains unknown how they are formed. In immunofluorescence microscopy, the immunofluorescence signal for the cell adhesion molecule nectin‐1 was concentrated on mitral cell lateral dendrites in the EPL of the developing mouse olfactory bulb. In electron microscopy, the immunogold particles for nectin‐1 were symmetrically localized on the plasma membranes at the contacts between mitral cell lateral dendrites, which showed bilateral darkening without dense cytoskeletal undercoats characteristic of puncta adherentia junctions. We named the contacts where the immunogold particles for nectin‐1 were symmetrically accumulated "nectin‐1 spots." The nectin‐1 spots were 0.21 μm in length on average and the distance between the plasma membranes was 20.8 nm on average. In 3D reconstruction of serial sections, clusters of the nectin‐1 spots formed a disc‐like structure. In the mitral cell lateral dendrites of nectin‐1‐knockout mice, the immunogold particles for nectin‐1 were undetectable and the plasma membrane darkening was electron‐microscopically normalized, but the plasma membranes were partly separated from each other. The nectin‐1 spots were further identified between mitral cell lateral and primary dendrites and between mitral cell lateral dendrites and granule cell dendritic spine necks. These results indicate that the nectin‐1 spots constitute a novel adhesion apparatus that tethers mitral cell dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb. J. Comp. Neurol. 523:1824–1839, 2015. © 2015 Wiley Periodicals, Inc.