In situ relationships between microbiota and potential pathobiota in Arabidopsis thaliana
In situ relationships between microbiota and potential pathobiota in Arabidopsis thaliana
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DOI：
10.1038/s41396-018-0152-7
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发表时间：
2018-08-01
期刊：
影响因子：
11
通讯作者：
Roux, Fabrice
中科院分区：
文献类型：
--
作者：
Bartoli, Claudia;Frachon, Lea;Roux, Fabrice
微生物发病机制中的当前挑战是鉴定可以防止和/或限制微生物病原体对宿主的入侵的生物控制剂。在自然界中，宿主常常被多种病原体感染。然而，目前大多数研究都是在实验室控制条件下进行的，并考虑了单一病原菌种属和单一病原菌种属之间的相互作用。因此，下一步是在现实的生态环境中探索宿主微生物群落（微生物群）和具有潜在致病行为的微生物成员（致病生物群）之间的关系。在本研究中，我们调查了163个生态对比的拟南芥种群在法国西南部的两个季节采样的根相关和叶相关的细菌群落内的这种关系。与入侵悖论的理论相一致，我们观察到微生物和病原生物群α多样性之间存在显著的驼峰关系，这种关系在季节和植物器官之间都很强大。在大多数人群中，我们还观察到季节之间微生物组成的强烈动态。因此，潜在的致病生物群的组成解释的组合季节特异性微生物群的操作分类单位。这一结果表明，控制病原体入侵的潜在生物标志物是高度动态的。
A current challenge in microbial pathogenesis is to identify biological control agents that may prevent and/or limit host invasion by microbial pathogens. In natura, hosts are often infected by multiple pathogens. However, most of the current studies have been performed under laboratory controlled conditions and by taking into account the interaction between a single commensal species and a single pathogenic species. The next step is therefore to explore the relationships between host-microbial communities (microbiota) and microbial members with potential pathogenic behavior (pathobiota) in a realistic ecological context. In the present study, we investigated such relationships within root-associated and leaf-associated bacterial communities of 163 ecologically contrasted Arabidopsis thaliana populations sampled across two seasons in southwest of France. In agreement with the theory of the invasion paradox, we observed a significant humped-back relationship between microbiota and pathobiota alpha-diversity that was robust between both seasons and plant organs. In most populations, we also observed a strong dynamics of microbiota composition between seasons. Accordingly, the potential pathobiota composition was explained by combinations of season-specific microbiota operational taxonomic units. This result suggests that the potential biomarkers controlling pathogen's invasion are highly dynamic.