INTERACTIONS OF VARIOUS SKELETAL INTRACRYSTALLINE COMPONENTS WITH CALCITE CRYSTALS
INTERACTIONS OF VARIOUS SKELETAL INTRACRYSTALLINE COMPONENTS WITH CALCITE CRYSTALS
复制标题
DOI：
10.1021/ja00078a005
复制
发表时间：
1993-12-15
影响因子：
15
通讯作者：
WEINER, S
中科院分区：
文献类型：
--
作者：
ALBECK, S;AIZENBERG, J;WEINER, S
许多生物体的方解石骨骼元素含有少量的蛋白质和糖蛋白，它们被结合在方解石的单晶中。提取和部分纯化的晶内大分子允许识别它们与合成方解石晶体的相互作用。特定的相互作用推断诱导的形态学修改与这些大分子在溶液中的存在下生长的方解石晶体上的新面孔的发展。从海胆刺中提取的部分纯化的大分子仅与大致平行于方解石c晶轴的面相互作用，产生发育良好的（01 l）面。 从软体动物壳棱柱中提取的大分子被分成两个部分，每个部分具有特征性的氨基酸组成，并显示出与生长中的方解石晶体的不同的特异性相互作用：一个高度酸性的部分与{001}面集相互作用，而一个不同的部分，它只是中等酸性，具有类似于海胆刺的氨基酸组成，具体地与{01 l}面集合交互。镁，海胆刺的主要离子成分，导致{011}晶面组的发展。在生物晶体中的大分子的嵌入方向上的现有知识与合成晶体中的相互作用平面一致，这表明我们的体外测定与体内系统的相关性。我们认为，每一个单独的相互作用模式可以推断在生物晶体生长的调节中的特定作用。
The calcitic skeletal elements of many organisms contain small amounts of proteins and glycoproteins which are incorporated within single crystals of calcite. Extraction and partial purification of the intracrystalline macromolecules allowed the identification of their interactions with synthetic calcite crystals. Specific interactions were inferred from induced morphological modifications associated with the development of new faces on calcite crystals grown in the presence of these macromolecules in solution. Partially purified macromolecules extracted from within sea urchin spines interacted only with faces roughly parallel to the c crystallographic axis of calcite, producing well-developed (01l} faces. Macromolecules extracted from within mollusk shell prisms separated into two fractions each having a characteristic amino acid composition and displayed distinct specific interactions with growing calcite crystals: A highly acidic fraction interacts with the {001} set of faces, while a different fraction, which is only moderately acidic and has an amino acid composition similar to that of the sea urchin spines, interacts specifically with the {01l} set of faces. Magnesium, a major ionic component of the sea urchin spines, causes the development of the {011} set of crystal faces. The existing knowledge on the direction of intercalation of the macromolecules in the biogenic crystals agrees with the interacting planes in the synthetic crystals, suggesting the relevance of our in vitro assay to the system in vivo. We suggest that each separate mode of interaction may infer a specific role in the regulation of biological crystal growth.