Recent habitat fragmentation due to roads can lead to significant genetic differentiation in an abundant flightless ground beetle
Recent habitat fragmentation due to roads can lead to significant genetic differentiation in an abundant flightless ground beetle
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DOI：
10.1111/j.1365-294x.2004.02310.x
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发表时间：
2004-10-01
影响因子：
4.9
通讯作者：
Largiadèr, CR
中科院分区：
文献类型：
--
作者：
Keller, I;Nentwig, W;Largiadèr, CR
虽然栖息地破碎化被怀疑对生物多样性构成重大威胁，但其对丰富的无脊椎动物物种的影响却很少被调查。我们评估了遗传种群结构的不会飞的地面甲虫Abax parallelepipedus在两个主要道路和一条高速公路使用5个微卫星位点的森林碎片。我们检测到低水平的遗传分化，这是一致的高人口密度的632-1707个人/公顷的标记重捕方法估计。曼特尔测试检测到一个非常显着的增加，成对的F-ST值与抽样地点之间的道路数量。正如预期的那样，在最小片段（高速公路出口环路）的样本中观察到了道路隔离的最显著影响，该样本与大多数其他位置有显著差异。然而，没有迹象表明，最近的瓶颈或遗传变异的损失，在这个群体中检测到，表明仍然相对较大的有效人口规模（N-E）。计算机模拟证实，所观察到的F-ST值确实与该片段中几百个个体的N-e相一致，假设自道路建设以来存在强烈或绝对的隔离。我们讨论了我们的研究结果的影响，丰富的，但分散在破碎的栖息地物种的保护。
Although habitat fragmentation is suspected to pose a major threat to biodiversity, its impact on abundant invertebrate species has been little investigated. We assessed the genetic population structure of the flightless ground beetle Abax parallelepipedus in a forest fragmented by two main roads and a highway using five microsatellite loci. We detected low levels of genetic differentiation, which was concordant with the high population densities of 632-1707 individuals/ha estimated with a mark-recapture method. A Mantel test detected a highly significant increase of pairwise F-ST-values with the number of roads between sampling locations. As expected, the most pronounced effect of the isolation due to roads was observed in the sample from the smallest fragment (highway exit loop), which was differentiated significantly from most other locations. However, no signs of a recent bottleneck or a loss of genetic variability were detected in this population, indicating a still relatively large effective population size (N-e). Computer simulations confirmed that the observed F-ST-values were indeed compatible with a N-e of a few hundred individuals in this fragment, assuming strong or absolute isolation since the construction of the roads. We discuss the implications of our findings for the conservation of abundant but poorly dispersing species in fragmented habitats.