ARTICULAR CARTILAGE OPTICAL PROPERTIES IN THE SPECTRAL RANGE 300-850 NM

ARTICULAR CARTILAGE OPTICAL PROPERTIES IN THE SPECTRAL RANGE 300-850 NM
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DOI:
10.1117/1.429893
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发表时间:
1998-07-01
影响因子:
3.5
通讯作者:
Bertone, Alicia L.
Bertone, Alicia L.
中科院分区:
医学3区
文献类型:
--
作者:
Ebert, Daniel W.;Roberts, Cynthia;Bertone, Alicia L.

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使用带有积分球附件的分光光度计,从承重(n=9)和非承重(n=9)马关节软骨标本记录300至850 nm的绝对全反射率测量值。根据界面反射和边缘损失对测得的光谱进行校正后,应用Kubelka-Munk理论估计吸收和散射系数、一维光强分布和光穿透深度。Kubelka-Munk吸收系数范围从330 nm处的7 cm(-1)到850 nm处的1 cm(-1)。在340 nm处观察到局部吸收峰。在510 nm以上,负重软骨的吸收系数明显高于非负重组织(配对t检验,p
Measurements of absolute total reflectance were recorded from weight-bearing (n=9) and nonweight-bearing (n=9) equine articular cartilage specimens from 300 to 850 nm using a spectrophotometer with integrating sphere attachment. Following correction of measured spectra for interfacial reflections and edge losses, Kubelka-Munk theory was applied to estimate absorption and scattering coefficient, one-dimensional light intensity distribution, and light penetration depth. Kubelka-Munk absorption coefficients ranged from similar to 7 cm(-1) at 330 nm to similar to 1 cm(-1) at 850 nm. A localized absorption peak was noted at; 340 nm. Above 510 nm, weight-bearing cartilage demonstrated significantly higher absorption coefficients than nonweight-bearing tissue (paired t-test, p