Accelerating the start-up of the cathodic biofilm by adding acyl-homoserine lactone signaling molecules
Accelerating the start-up of the cathodic biofilm by adding acyl-homoserine lactone signaling molecules
复制标题
通过添加酰基高丝氨酸内酯信号分子加速阴极生物膜的启动
DOI：
10.1016/j.biortech.2018.07.095
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发表时间：
2018-10-01
影响因子：
11.4
通讯作者：
Zhou, Shungui
中科院分区：
文献类型：
--
作者：
Fang, Yanlun;Deng, Chengsheng;Zhou, Shungui
电活性生物膜（EAB）对于生物电化学系统至关重要，然而，阴极EAB的形成比阳极EAB更耗时。本研究旨在评估酰基高丝氨酸内酯 (AHL) 是否可以促进阴极土杆菌 EAB 的启动。添加 AHL 后，阴极相关 G. soli EAB 的生物量、细胞活力和胞外聚合物 (EPS) 丰度均有所增加。同样，在 AHL 存在下，阴极 EAB 中 EPS 和最外层蛋白质的氧化还原活性得到增强，从而导致生物膜更好的启动性能。与未添加 AHL 的对照相比，在启动周期中添加 AHL 后，启动滞后期缩短了约 50%，电子吸收增强了 1.3-2.0 倍，反硝化率提高了一倍多，与成熟的 G. soli 阴极 EAB 相当。这些发现为通过 AHL 加速阴极生物膜的启动提供了机会。
Electroactive biofilms (EABs) are essential for bioelectrochemical systems, however, the formation of cathodic EABs is more time-consuming than anodic EABs. This study aims to evaluate whether acyl-homoserine lactones (AHLs) could facilitate the start-up of cathodic Geobacter soli EABs. With AHL addition, the biomass, cell viability, and extracellular polymeric substance (EPS) abundance of cathode-associated G. soli EABs were increased. Likewise, redox activities of EPS and outermost proteins in the cathodic EABs were enhanced in the presence of AHLs, which consequently led to better start-up performance of biofilms. Compared to the control without AHLs, start-up lag periods were reduced by approximately 50%, electron uptakes were enhanced by 1.3-2.0 times, and denitrification rates were more than doubled with AHL addition in the start-up cycle, which were comparable to those of mature G. soli cathodic EABs. These findings open up an opportunity for accelerating the start-up of cathodic biofilms via AHLs.