HISTOPATHOLOGICAL STUDY OF PROTECTIVE IMMUNITY AGAINST MURINE SALMONELLOSIS INDUCED BY KILLED VACCINE
HISTOPATHOLOGICAL STUDY OF PROTECTIVE IMMUNITY AGAINST MURINE SALMONELLOSIS INDUCED BY KILLED VACCINE
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DOI：
10.1128/iai.39.1.423-430.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
HSU, HS
中科院分区：
文献类型：
--
作者：
NAKONECZNA, I;HSU, HS
瑞士韦伯斯特小鼠接种了热杀灭沙门氏菌，然后感染了致命的鼠伤寒沙门氏菌。18只接种疫苗的老鼠中只有1只在104次的攻击中死亡。LD50;.apprx。70%的人在105次的挑战中幸存下来。LD50。在这些接种疫苗的小鼠中发生的病变的组织病理学检查显示了小鼠沙门氏菌病原发性病变的特征。感染后6-7天内出现早期坏死伴多形核白细胞浸润及脓肿形成。这些脓肿仍然小而分散。到第7-10天，病变开始转变为肉芽肿，首先出现外周单核细胞，然后是多形细胞的替换。感染第3周时，在脾脏、肝脏和淋巴结可见散在的微小离散肉芽肿。随后是愈合和组织再生。因此，接种热杀沙门氏菌的小鼠发生的传染性病变的特征与接种无毒疫苗的小鼠发生的病变明显不同。
Swiss-Webster mice were vaccinated with heat-killed salmonellae and then were infected with virulent Salmonella typhimurium. Only 1 of 18 vaccinated mice died from a challenge of 104 .times. LD50; .apprx. 70% survived a challenge of 105 .times. LD50. Histopathological examinations of the lesions developed in these vaccinated mice showed the characteristic features of a primary lesion in murine salmonellosis. There was an early necrosis with infiltration of polymorphonuclear leukocytes and abscess formation within the first 6-7 days after infection. These abscesses remained small and discrete. By days 7-10, the lesions began to transform into granulomas, first with the appearance of peripheral mononuclear cells and then by the replacement of polymorphs. By the 3rd wk of the infection, minute and discrete granulomas were seen scattered in the spleen, liver and lymph nodes. Healing and tissue regeneration followed. Thus, the characteristics of infectious lesions developed in mice vaccinated with heat-killed salmonellae are distinctly different from those developed in mice protected by the avirulent vaccine.