High-sensitivity negative-ion laser spray for liquid chromatography/mass spectrometry.
High-sensitivity negative-ion laser spray for liquid chromatography/mass spectrometry.
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用于液相色谱/质谱分析的高灵敏度负离子激光喷雾。
DOI：
10.1002/rcm.473
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Ueda
中科院分区：
文献类型：
--
作者：
K. Hiraoka;Y. Asakawa;Y. Yamamoto;M. Nakamura;K. Ueda
研制了一种用于液相色谱/质谱联用的负离子模式激光喷雾接口。对于除糖之外的所有研究样品，激光喷雾的灵敏度比负离子电喷雾高出几个数量级。激光喷雾胆固醇3-硫酸钠水溶液形成碎片离子HSO（-4）和SO（-3）。这表明，结构信息可以直接从激光喷雾质谱热不稳定的化合物。
A negative-ion mode laser spray interface for use in liquid chromatography/mass spectrometry (LC/MS) has been developed and investigated. The laser spray gave sensitivities that were orders of magnitude better than the negative-ion electrospray for all samples investigated with the exception of sugars. Fragment ions, HSO(-4)and SO(-3), were formed from the laser-sprayed aqueous solution of cholesterol 3-sulfate sodium salt. This suggests that structural information may be obtained directly from the laser-spray mass spectra for thermally labile compounds.