Histamine excites rat lateral vestibular nuclear neurons through activation of post-synaptic H2 receptors
Histamine excites rat lateral vestibular nuclear neurons through activation of post-synaptic H2 receptors
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DOI：
10.1016/j.neulet.2008.10.027
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发表时间：
2008-12
影响因子：
2.5
通讯作者：
Jun Zhang;Xiao‐Hu Han;Hong‐Zhao Li;Jing-Ning Zhu;Jian-jun Wang
中科院分区：
文献类型：
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作者：
Jun Zhang;Xiao‐Hu Han;Hong‐Zhao Li;Jing-Ning Zhu;Jian-jun Wang
通过脑干脑片全细胞膜片记录，观察组胺对前庭外侧核(LVN)神经元活动的影响。沐浴组胺可引起自发放电(n=19)和静息(n=7)LVN神经元的浓度依赖性兴奋。此外，组胺在LVN神经元上产生稳定的内向电流(n=5)，在河豚毒素存在下(n=4)，组胺引起的膜电位去极化持续存在，提示组胺对LVN神经元具有直接突触后效应。选择性组胺H2受体拮抗剂雷尼替丁能有效阻断组胺所致的兴奋性反应(n=4)，而选择性组胺H1受体拮抗剂曲普利定不能阻断组胺诱发的兴奋性反应(n=4)。此外，选择性组胺H2受体激动剂DIMAPRIT(n=3)而不是选择性组胺H1受体激动剂2-吡啶乙胺(n=4)可模拟组胺对LVN神经元的兴奋作用。结果表明，组胺通过突触后组胺H2受体兴奋LVN神经元，提示来自下丘脑的中枢组胺能投射可能调节LVN神经元的活动，并积极影响前庭反射和功能。
Through whole-cell patch recordings in brainstem slices, the effects of histamine on neuronal activity of the lateral vestibular nucleus (LVN) were investigated. Bath application of histamine elicited a concentration-dependent excitation of both spontaneous firing (n=19) and silent (n=7) LVN neurons. Moreover, histamine induced a stable inward current in the LVN neurons (n=5) and the histamine-induced depolarization of membrane potential persisted in the presence of tetrodotoxin (n=4), indicating a direct post-synaptic effect of the histamine on the LVN neurons. Selective histamine H2 receptor antagonist ranitidine effectively blocked the histamine-evoked excitatory responses on the LVN neurons (n=4), but selective histamine H1 receptor antagonist triprolidine did not (n=4). In addition, selective histamine H2 receptor agonist dimaprit (n=3) rather than 2-pyridylethylamine (n=4), a selective histamine H1 receptor agonist, mimicked the excitatory action of histamine on LVN neurons. The results demonstrate that histamine excites the LVN neurons via post-synaptic histamine H2 receptors and suggest that the central histaminergic projection arising from the hypothalamus may modulate LVN neurons activity and actively influence the vestibular reflexes and functions.