Effects of epidermal growth factor, estrogen, and progestin on DNA synthesis in mammary cells in vivo are determined by the developmental state of the gland.
Effects of epidermal growth factor, estrogen, and progestin on DNA synthesis in mammary cells in vivo are determined by the developmental state of the gland.
复制标题
表皮生长因子、雌激素和孕激素对体内乳腺细胞 DNA 合成的影响取决于腺体的发育状态。
DOI：
10.1002/jcp.1041550110
复制
发表时间：
1993
影响因子：
5.6
通讯作者：
Nummy,KA
中科院分区：
文献类型：
--
作者：
Haslam,SZ;Counterman,LJ;Nummy,KA
雌激素(E)、孕激素(P)和表皮生长因子（EGF）参与正常乳腺的生长发育。虽然已经开展了一些研究来研究EGF在未成熟乳腺中的体内作用，但对于EGF在成年乳腺中的生长作用及其与E和/或P的潜在相互作用尚不清楚。本研究旨在探讨EGF、E和P对未成熟、青春期周边和成年、性成熟小鼠乳腺细胞增殖的影响。我们发现EGF促进上皮细胞和基质细胞在未成熟和成年乳腺中的增殖。在未成熟腺体中，终芽上皮对EGF的增殖作用最敏感，EGF、E和/或P之间没有明显的相互作用。相反，在成熟腺体中，EGF增加了导管上皮中E+P的增殖作用，导致更广泛的导管侧分支。因此，这些结果表明乳腺的发育状态决定了EGF、E和P在生长发育过程中相互作用的性质和程度。©1993 Wiley‐Liss, Inc。
Estrogen (E), progesterone (P), and epidermal growth factor (EGF) are involved in the growth and development of the normal mammary gland. While studies have been carried out to investigate the in vivo effects of EGF in the immature mammary gland, nothing is known about the growth effects of EGF or its potential interactions with E and/or P in the adult mammary gland. The present studies were undertaken to investigate the effects of EGF, E, and P on mammary cell proliferation in immature, peripubertal vs. adult, sexually mature mice. We have found that EGF promotes epithelial and stromal cell proliferation in both the immature and adult mammary glands. In the immature gland, the end bud epithelium is most responsive to the proliferative effects of EGF and there is no apparent interaction between EGF, E, and/or P. In contrast, in the mature gland EGF adds to the proliferative effects of E+P in the ductal epithelium resulting in more extensive ductal sidebranching. Thus these results demonstrate that the developmental state of the mammary gland determines the nature and extent of the interactions between EGF, E, and P in growth and development. © 1993 Wiley‐Liss, Inc.