Electrochemical integrated paper-based immunosensor modified with multi-walled carbon nanotubes nanocomposites for point-of-care testing, of 17 beta-estradiol
Electrochemical integrated paper-based immunosensor modified with multi-walled carbon nanotubes nanocomposites for point-of-care testing, of 17 beta-estradiol
复制标题
用多壁碳纳米管纳米复合材料修饰的电化学集成纸基免疫传感器，用于 17 β-雌二醇的即时检测
DOI：
10.1016/j.bios.2018.02.012
复制
发表时间：
2018
影响因子：
12.6
通讯作者：
Cai Xinxia
中科院分区：
文献类型：
--
作者：
Wang Yang;Luo Jinping;Liu Juntao;Li Xinrong;Kong Zhuang;Jin Hongyan;Cai Xinxia
17β-estradiol (17β-E2) plays a critical role in regulating reproduction in human, there is therefore an urgent need to detect it sensitively and precisely in a cost-effective and easy method. In this paper, a label-free integrated microfluidic paper-based analytical device was developed for highly sensitive electrochemical detection of 17β-E2. The microfluidic channel of the paper-based sensor was fabricated with wax printing and the three electrodes, including working, counter and reference electrode were screen-printed. Multi-walled carbon nanotubes (MWCNTs)/ thionine (THI)/ gold nanoparticles (AuNPs) Nano composites were synthesized and coated on screen-printed working electrode (SPWE) for the immobilization of anti-E2. In this electro-chemical system of paper-based immunoassay, THI molecules serving as an electrochemical mediator while MWCNTs and AuNPs, due to their excellent electrical conductivities, could accelerate electron transfer for the signal amplification. Experimental results revealed that the immunoassay is able to detect 17β-E2 as low as 10 pg mL−1, with a linear range from 0.01 to 100 ng mL−1. This microfluidic paper-based immunosensor would provide a new platform for low cost, sensitive, specific, and point-of-care diagnosis of 17β-E2.
