Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin.
Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin.
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通过荧光团 Zinquin 评估附睾成熟过程中小鼠精子中不稳定锌分布的变化。
DOI：
10.1071/rd9961097
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
F. Sun
中科院分区：
文献类型：
--
作者：
P. Zalewski;X. Jian;L. Soon;W. Breed;R. Seamark;S. Lincoln;A. D. Ward;F. Sun
采用Zn（II）特异性荧光团Zinquin测定游离或松散结合Zn（II）在小鼠精子中的区域分布。来自睾丸的精子在整个头部显示出明亮的荧光；来自附睾头的通常在顶体后区域荧光更亮；附睾尾的精子荧光不太亮，荧光焦点在精子头部，用Triton X-100提取后消失，因此似乎是膜相关的。尾端精子用十二烷基硫酸钠处理后，在头部产生明亮均匀的锌质荧光，与在头形精子中观察到的相似，这表明两种类型的精子具有相似数量的头部锌（II），但锌（II）结合锌质的可用性不同。相比之下，来自男性生殖道不同区域的精子尾部荧光强度相似，并且在很大程度上不受Triton X-100提取的影响，这与细胞内位置一致。在大鼠的头端精子和尾端精子之间也观察到类似的差异。由此可见，精子亚细胞区室中游离或松散结合的Zn（II）的可视化和测量有助于研究这种金属在精子发育和功能中的作用，以及可能伴随不育和Zn（II）缺乏的异常。
The Zn(II)-specific fluorophore Zinquin was used to determine the regional distribution of free or loosely-bound Zn(II) in mouse spermatozoa. Spermatozoa from the testes exhibited bright fluorescence over the entire head; those from the caput epididymides generally fluoresced more brightly in the post-acrosomal region; and spermatozoa from the caudae epididymides fluoresced less brightly, with foci of fluorescence over the sperm head which were lost after extraction with Triton X-100 and hence appeared to be membrane-associated. Treatment of cauda sperm with sodium dodecyl sulfate resulted in a bright uniform Zinquin fluorescence in the heads, similar to that observed in caput sperm, indicating that the two types of sperm have similar amounts of head Zn(II) but that the availability of Zn(II) for binding Zinquin is different. By contrast, the intensity of tail fluorescence was similar in spermatozoa from different regions of the male reproductive tract and was largely unaffected by Triton X-100 extraction, consistent with an intracellular location. Similar differences were observed between caput sperm and cauda sperm in the rat. It is concluded that visualization and measurement of free or loosely-bound Zn(II) in subcellular compartments of spermatozoa should facilitate investigation of the role of this metal in the development and function of spermatozoa and abnormalities that might accompany infertility and Zn(II) deficiency.