Detection of c‐fms protooncogene in early mouse embryos by whole mount in situ hybridization indicates roles for macrophages in tissue remodelling
Detection of c‐fms protooncogene in early mouse embryos by whole mount in situ hybridization indicates roles for macrophages in tissue remodelling
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DOI：
10.1111/j.1365-2141.1995.tb05220.x
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发表时间：
1995-08
影响因子：
6.5
通讯作者：
D. Hume;S. Monkley;B. Wainwright
中科院分区：
文献类型：
--
作者：
D. Hume;S. Monkley;B. Wainwright
概括。通过全原位杂交将编码巨噬细胞集落刺激因子（CSF-1）受体的c-fms原癌基因定位于发育中的小鼠胚胎中，c-fms首先在胎盘滋养层细胞中表达。大约 9-5 dpc，在卵黄囊中可检测到分离的 c-/ms 阳性细胞，到 10-5 dpc 时，在整个胚胎中可检测到大量细胞。 c-fms 表达的定位与其对巨噬细胞的限制以及这些巨噬细胞在组织更新和广泛细胞死亡部位的位置一致。
Summary. The c‐fms protooncogene which encodes the receptor for macrophage colony‐stimulating factor (CSF‐1) was localized in the developing mouse embryo by whole in situ hybridization, c‐fms was expressed first in placental trophoblasts. Around 9‐5 dpc, isolated c‐/ms‐positive cells became detectable in the yolk sac and by 10‐5 dpc large numbers were detectable throughout the embryo. The localization of c‐fms expression was consistent with its restriction to macrophages, and with the location of those macrophages in sites of tissue turnover and extensive cell death.