Experimental autoimmune thyroiditis. In vitro cytotoxic effects of T lymphocytes on thyroid monolayers
Experimental autoimmune thyroiditis. In vitro cytotoxic effects of T lymphocytes on thyroid monolayers
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实验性自身免疫性甲状腺炎。
DOI：
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发表时间：
1983
影响因子：
15.3
通讯作者：
Noel R. Rose
中科院分区：
文献类型：
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作者：
Noel R. Rose
为探讨实验性自身免疫性甲状腺炎(EAT)的效应机制，建立了以甲状腺靶细胞为靶细胞的细胞毒体系。取8~10周龄CBA/J小鼠(8~10周龄)，在完全弗氏佐剂中加入120微克小鼠甲状腺球蛋白(MTG)(后足和大腿各0.2ml)，免疫12~18d后，取窝和腹股沟淋巴结细胞(LNC)，加入MTG(10~50微克/ml)培养。在培养第5天，将活的LNC加入标记的甲状腺单层，16h后检测其细胞毒作用，在培养液中加入1 mM二丁酰腺苷3‘，5’-环状单磷酸和60微U促甲状腺激素/ml，制备出功能正常的甲状腺靶细胞，以MTG生成反应最多9d。在第5-7天，用111In标记融合单层并作为靶点。特异性~(111)In释放率在56%~85%之间。这种细胞毒反应是MTG特异性的，H-2受到限制。用兔抗MTG血清处理甲状腺靶细胞，可完全抑制其细胞毒作用。用KK或DK产物的小鼠抗血清预处理后，抑制率约为50%，而两种抗血清联合使用则完全抑制。以对照BALB/c甲状腺细胞为靶细胞，未观察到细胞毒作用。研究了MTG对Thy-1+细胞毒作用的体外扩增动力学。细胞毒反应需要5d才能形成，第4天用抗Lyt-1.1的单抗处理LNC，但不能用Lyt-2.1加补体的单抗处理LNC，这种细胞毒反应被取消。相反，到了第5天，用Lyt-2.1的抗血清进行类似的处理后，细胞毒性被取消，但Lyt-1.1的抗血清没有。我们的结论是，MTG免疫小鼠的细胞毒细胞是Lyt-2阳性细胞，但它们的生成和/或分化需要Lyt-1阳性细胞的存在。
Effector mechanisms in experimental autoimmune thyroiditis (EAT) were studied in vitro by establishing a cytotoxicity system with thyroid target cells. Lymph node cells (LNC) from popliteal and inguinal lymph nodes were obtained from CBA/J mice (8-10 wk old) 12-18 d after immunization with 120 micrograms mouse thyroglobulin (MTg) in complete Freund's adjuvant (0.2 ml to both hind footpads and thighs) and were cultured with MTg (10-50 micrograms/ml). On day 5 of culture, viable LNC were added to labeled thyroid monolayers and their cytoxicity was assayed after 16 h. Functional thyroid target cells, as reflected by MTg production for up to 9 d, were prepared by adding 1 mM dibutyryl adenosine 3',5'-cyclic monophosphate and 60 microU thyroid-stimulating hormone/ml to the culture medium. On days 5-7, confluent monolayers were labeled with 111In and used as targets. Specific 111In-release ranged from 56 to 85%. The cytotoxic response is MTg specific and H-2 restricted. Pretreatment of thyroid target cells with rabbit antiserum to MTg completely inhibited cytotoxicity. Pretreatment with mouse antiserum to either Kk or Dk products resulted in approximately 50% inhibition, whereas the combined use of both antisera led to total inhibition. No cytotoxicity was observed when control BALB/c thyroid cultures were the target cells. The kinetics of the expansion of Thy-1+ cytotoxic cells by in vitro exposure to MTg were then studied. The cytotoxic response required 5 d to develop and was abolished by treating LNC on day 4 with monoclonal antibody to Lyt-1.1, but not to Lyt-2.1, plus complement. In contrast, by day 5, cytotoxicity was abrogated by similar treatment with antiserum to Lyt-2.1, but not to Lyt-1.1. We conclude that cytotoxic cells derived from MTg-immunized mice are Lyt-2-bearing cells but require the presence of Lyt-1-bearing cells for their generation and/or differentiation.
鸡自发性自身免疫性甲状腺炎。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Livezey,MD;Sundick,RS;Rose,NR
通讯作者：
Rose,NR