Elevated virus loads of Kaposi's sarcoma-associated human herpesvirus 8 predict Kaposi's sarcoma disease progression, but elevated levels of human immunodeficiency virus type 1 do not
Elevated virus loads of Kaposi's sarcoma-associated human herpesvirus 8 predict Kaposi's sarcoma disease progression, but elevated levels of human immunodeficiency virus type 1 do not
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DOI：
10.1086/340652
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发表时间：
2002-06-15
影响因子：
6.4
通讯作者：
Wehbie, RS
中科院分区：
文献类型：
--
作者：
Quinlivan, EB;Zhang, C;Wehbie, RS
卡波西肉瘤相关疱疹病毒 (KSHV) 存在于卡波西肉瘤 (KS)、多中心卡斯尔曼病和原发性渗出性淋巴瘤中。为了前瞻性地评估 KSHV 载量作为 AIDS 相关 KS 男性队列中 KS 临床状态和预后的生物标志物，开发并测试了 2 种定量聚合酶链反应 (PCR) 测定法，以确定 KSHV 外周血单核细胞 (PBMC) 病毒载量。通过定量竞争性 (QC) 和实时应用生物系统 (ABI) PCR 检测，大多数预后良好 KS 的患者 (13/15) 的 KSHV 拷贝数小于或等于 1.5 log KSHV 拷贝/10(5) PBMC。两种检测方法对于识别 20 个月随访期间没有 KS 进展的 16 名患者的特异性为 94%。 QC-PCR 和 ABI-PCR 对于识别 5 名 KS 进展的患者分别表现出 100% 和 80% 的诊断灵敏度。二分人类免疫缺陷病毒载量和二分 CD4 计数均不能预测 KS 进展或 KS 临床分期（所有阳性预测值
Kaposi's sarcoma-associated herpesvirus (KSHV) is found in Kaposi's sarcoma (KS), multicentric Castleman's disease, and primary effusion lymphomas. To prospectively evaluate KSHV load as a biomarker for KS clinical status and prognosis in a cohort of men with AIDS-related KS, 2 quantitative polymerase chain reaction (PCR) assays were developed and tested to determine KSHV peripheral blood mononuclear cell (PBMC) virus loads. Most patients (13/15) with good-prognosis KS had less than or equal to 1.5 log KSHV copies/10(5) PBMC by both quantitative competitive (QC) and real-time Applied Biosystems (ABI) PCR. Both assays provided 94% specificity for identifying the 16 patients without KS progression during 20 months of follow-up. QC-PCR and ABI-PCR exhibited 100% and 80% levels of diagnostic sensitivity, respectively, for identifying the 5 patients whose KS progressed. Neither dichotomized human immunodeficiency virus loads nor dichotomized CD4 counts predicted either KS progression or KS clinical stage (all positive predictive values