Combinatorial and chemotopic odorant coding in the zebrafish olfactory bulb visualized by optical imaging
Combinatorial and chemotopic odorant coding in the zebrafish olfactory bulb visualized by optical imaging
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DOI：
10.1016/s0896-6273(00)80314-1
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发表时间：
1997-05-01
期刊：
影响因子：
16.2
通讯作者：
Korsching, SI
中科院分区：
文献类型：
--
作者：
Friedrich, RW;Korsching, SI
气味被认为是由分布在嗅球(OB)中的肾小球模块的代码来表示的。在这里，我们用一种钙敏感染料标记初级传入神经后，光学成像了一类天然气味(氨基酸[AAs])诱导的斑马鱼OB肾小球模块中的突触前活动。AAS诱导活性肾小球模块的复杂组合模式，这些组合模式对不同刺激和浓度是独特的。定量分析表明，明确的刺激分子特征与空间受限的肾小球模块组中的活性相关。这些结果提供了直接证据，表明气味的身份和浓度是由肾小球活动模式编码的，并揭示了肾小球模块阵列的粗略化学组织。
Odors are thought to be represented by a distributed code across the glomerular modules in the olfactory bulb (OB). Here, we optically imaged presynaptic activity in glomerular modules of the zebrafish OB induced by a class of natural odorants (amino acids [AAs]) after labeling of primary afferents with a calcium-sensitive dye. AAs induce complex combinatorial patterns of active glomerular modules that are unique for different stimuli and concentrations. Quantitative analysis shows that defined molecular features of stimuli are correlated with activity in spatially confined groups of glomerular modules. These results provide direct evidence that identity and concentration of odorants are encoded by glomerular activity patterns and reveal a coarse chemotopic organization of the array of glomerular modules.