Surface modification of imprinted polymer microspheres with ultrathin hydrophilic shells to improve selective recognition of glutathione in aqueous media.
Surface modification of imprinted polymer microspheres with ultrathin hydrophilic shells to improve selective recognition of glutathione in aqueous media.
复制标题
DOI：
10.1016/j.msec.2015.11.018
复制
发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Renyuan Song;Xiaoling Hu;Ping Guan;J. Li;Chunbao Du;Liwei Qian;Chaoli Wang
中科院分区：
文献类型：
--
作者：
Renyuan Song;Xiaoling Hu;Ping Guan;J. Li;Chunbao Du;Liwei Qian;Chaoli Wang
描述了一种解决疏水分子印迹聚合物（MIP）微球经典缺陷的通用、有效的方法。以带电荷单体甲基丙烯酸和不带电荷单体N-异丙基丙烯酰胺为亲水性功能单体，通过可控表面接枝聚合法合成了两种具有亲水性壳层的水溶性分子印迹聚合物微球，并将其用于水介质中谷胱甘肽的识别。采用扫描电镜、傅里叶变换红外光谱和接触角测定等方法对水溶性MIP微球的形貌和化学特征进行了表征。通过静态结合实验研究了它们的选择性识别性能，并与未接枝的MIP微球进行了比较。结果表明，两种水溶性MIP微球均能有效降低非特异性结合，显著提高印迹因子，其中以N-异丙基丙烯酰胺为单体制备的水溶性MIP微球具有更显著的识别性能。此外，通过调节辐照时间，可以很好地控制表面接枝亲水层的厚度，从而获得优异的识别性能。最后，所制备的水相容性MIP微球作为固相萃取材料的适用性进行了研究，通过竞争性结合试验，使用谷胱甘肽及其类似物的混合物。
A universal, effective approach addressing the classical limitations of hydrophobic molecularly imprinted polymer (MIP) microspheres was described. Two water-compatible MIP microspheres with ultrathin hydrophilic shells were synthesized by controllable surface-graft polymerization using a charged monomer (methacrylic acid) and uncharged monomer (N-isopropylacrylamide) as the hydrophilic functional monomers for the recognition of glutathione in the aqueous medium. The morphological and chemical characteristics of the as-prepared water-compatible MIP microspheres were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy and contact angle measurements. Their selective recognition properties were investigated by static binding tests and compared with those of the ungrafted MIP microspheres. The results of this study showed that the both as-prepared water-compatible MIP microspheres effectively decreased non-specific binding and enhanced the imprinting factor significantly, and the water-compatible MIP microspheres prepared using N-isopropylacrylamide as monomer exhibited a more remarkable recognition property. In addition, the thickness of surface-grafted hydrophilic layer was well controlled by adjusting the irradiation time to obtain the excellent recognition property. Finally, the applicability of the as-prepared water-compatible MIP microspheres as solid-phase extraction materials was investigated by competitive binding tests using a mixture of glutathione and its analogs.