Prevalence of Enterocytozoon bieneusi in swine:: an 18-month survey at a slaughterhouse in Massachusetts
Prevalence of Enterocytozoon bieneusi in swine:: an 18-month survey at a slaughterhouse in Massachusetts
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DOI：
10.1128/aem.68.5.2595-2599.2002
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发表时间：
2002-05-01
影响因子：
4.4
通讯作者：
Tzipori, S
中科院分区：
文献类型：
--
作者：
Buckholt, MA;Lee, JH;Tzipori, S
采用聚合酶链反应（PCR）方法对屠宰场生猪的双胞虫感染情况进行了分析。32%的人被发现是阳性的，在夏季的几个月里这一比例更高。3株猪分离株的核糖体内转录间隔序列与人D型比氏埃希菌相同，2株与F型（来自猪）相同，9株以前未报道。这些孢子的生存能力通过它们感染非生菌仔猪的能力得到证实。原位杂交显示肝脏感染的存在。
Slaughterhouse pig samples were analyzed by PCR for Enterocytozoon bieneusi infection. Thirty-two percent were found to be positive, with rates being higher over the summer months. Three isolates from pigs were identical in their ribosomal internal transcribed spacer sequence to human E. bieneusi type D, two were identical to type F (from a pig), and nine were previously unreported. The viability of these spores was demonstrated by their ability to infect gnotobiotic piglets. The presence of the infection in liver was shown by in situ hybridization.