A role for Xlim-1 in pronephros development in Xenopus laevis
A role for Xlim-1 in pronephros development in Xenopus laevis
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DOI：
10.1006/dbio.2000.9951
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发表时间：
2000-12-15
影响因子：
2.7
通讯作者：
Asashima, M
中科院分区：
文献类型：
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作者：
Chan, TC;Takahashi, S;Asashima, M
Xlim-1是非洲爪蟾中表达的LIM类同源异型盒基因，是已知最早的原肾发育标记基因之一，在原肾雏形中表达。在这项研究中，我们研究了Xlim-1在前肾发育中的作用。使用RT-PCR分析在一系列诱导测定中分析外植体中Xlim-1的时间表达。Xlim-1表达9至15小时后，激活素/视黄酸处理，对应于原肾分化外植体。我们使用一系列显微注射实验进一步研究了Xlim-1的作用。将不同的Xlim-1突变体注射到假定的胚胎原肾原基中，通过RT-PCR和免疫组织化学分析这些Xlim-1突变体对胚胎和外植体原肾发生的影响。显性负性Xlim-1抑制活化素/视黄酸处理的动物帽中原肾的分化。在注射显性阴性形式的Xlim-1的胚胎中，前肾小管的发育在尾芽晚期受到抑制。我们的研究结果表明，Xlim-1可能不会启动分化的原肾，但它是必要的生长和伸长的原肾小管的发展。(C)北京大学出版社.
Xlim-1, a LIM class homeobox gene expressed in Xenopus laevis, is one of the earliest known marker genes of pronephros development and is expressed in pronephros rudiment. In this study, we examined the role of Xlim-1 in pronephros development. Temporal expression of Xlim-1 in explants was analyzed in a series of induction assays using RT-PCR analysis. Xlim-1 was expressed 9 to 15 h after activin/retinoic acid treatment, corresponding to pronephros differentiation in explants. We further examined the role of Xlim-1 using a series of microinjection experiments. Presumptive pronephric anlagen of embryos were injected with various Xlim-1 mutants, and effects of these Xlim-1 mutants on pronephrogenesis in embryos and in explants were analyzed by RT-PCR and immunohistochemistry. Dominant-negative Xlim-1 inhibited differentiation of pronephros in activin/retinoic acid-treated animal caps. In embryos injected with a dominant-negative form of Xlim-1, development of pronephric tubules was inhibited at the late tail-bud stage. Our results suggest that Xlim-1 may not initiate differentiation of the pronephros, but that it is necessary for growth and elongation in the development of pronephric tubules. (C) 2000 Academic Press.