Amniogenesis occurs in two independent waves in primates.
Amniogenesis occurs in two independent waves in primates.
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DOI：
10.1016/j.stem.2022.03.014
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发表时间：
2022-05-05
期刊：
影响因子：
23.9
通讯作者：
Rugg-Gunn, Peter J.
中科院分区：
文献类型：
--
作者：
Rostovskaya, Maria;Andrews, Simon;Reik, Wolf;Rugg-Gunn, Peter J.
在灵长类动物中，羊膜在着床过程中通过上胚层的空化而出现，而在其他物种中，羊膜在原肠胚形成后期通过外胚层的折叠而出现。在进化过程中，再生机制是如何多样化的仍然未知。出乎意料的是，灵长类动物胚胎的单细胞分析发现了两个转录和时间上不同的胚胎发生波。为了研究这一点，我们采用了人类多能干细胞（hPSC）的幼稚到引发的转变来模拟围植入期上胚层发育。部分致敏的hPSC瞬时获得分化成在转录和形态学上与早期羊膜匹配的空化上皮的能力，而完全致敏的hPSC产生类似于晚期羊膜的细胞，从而重现了两个独立的分化波。早期波遵循滋养外胚层样途径，包括空化，而后期波类似于原肠胚形成过程中的外胚层样途径。两个独立的波的发现解释了如何通过空化作用在进化过程中通过复制预先存在的滋养外胚层程序来实现再生。在灵长类动物中，羊膜的发育经历了两个不同的外胚层分化波。羊膜腔在早期通过滋养外胚层样途径形成。晚期羊膜发生遵循非神经外胚层样转录程序。不同状态的hPSC分化模拟了羊膜发生的两个波。人类围着床期发育期间的谱系特化仍然知之甚少。罗斯托夫斯卡娅等人发现在灵长类动物胚胎中，羊膜是在两个转录和时间上不同的外胚层分化波中形成的，并建立了一个基于人类多能干细胞的模型，该模型密切概括了两个外胚层分化波的时间和形态发生。
In primates, the amnion emerges through cavitation of the epiblast during implantation, whereas in other species it does so later at gastrulation by the folding of the ectoderm. How the mechanisms of amniogenesis diversified during evolution remains unknown. Unexpectedly, single-cell analysis of primate embryos uncovered two transcriptionally and temporally distinct amniogenesis waves. To study this, we employed the naive-to-primed transition of human pluripotent stem cells (hPSCs) to model peri-implantation epiblast development. Partially primed hPSCs transiently gained the ability to differentiate into cavitating epithelium that transcriptionally and morphologically matched the early amnion, whereas fully primed hPSCs produced cells resembling the late amnion instead, thus recapitulating the two independent differentiation waves. The early wave follows a trophectoderm-like pathway and encompasses cavitation, whereas the late wave resembles an ectoderm-like route during gastrulation. The discovery of two independent waves explains how amniogenesis through cavitation could emerge during evolution via duplication of the pre-existing trophectoderm program. The amnion develops in two distinct waves of epiblast differentiation in primates The amniotic cavity is formed during the early wave via a trophectoderm-like route Late amniogenesis follows a nonneural ectoderm-like transcriptional program Differentiation of hPSC from different states models the two waves of amniogenesis Lineage specification during human peri-implantation development remains poorly understood. Rostovskaya et al. discovered that in primate embryos the amnion is formed in two transcriptionally and temporally distinct waves of epiblast differentiation and established a human pluripotent stem cell-based model that closely recapitulates the timing and morphogenesis of both amniogenesis waves.
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