Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis
Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis
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DOI：
10.1128/iai.59.8.2653-2657.1991
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发表时间：
1991-08
影响因子：
3.1
通讯作者：
G R Riviere;K. S. Weisz;L G Simonson;S. A. Lukehart
中科院分区：
文献类型：
--
作者：
G R Riviere;K. S. Weisz;L G Simonson;S. A. Lukehart
本研究的目的是确定是否对梅毒螺旋体亚种病原体限制性抗原的单克隆抗体。苍白球可作为急性坏死性溃疡性牙龈炎患者患病牙龈组织中螺旋体的探针。生物素-链霉亲和素系统用于鉴定组织冷冻切片中单克隆抗体结合的螺旋体。来自患病部位的16份组织样本中有12份与病原体限制性抗体反应，但来自健康部位的8份组织样本均未与病原体限制性抗体反应。在溃疡附近的完整上皮和结缔组织中发现微生物。染色强度通常在血管周围位置和囊泡间隙周围较高。牙龈拟杆菌和齿垢密螺旋体的单克隆抗体分别与患病牙龈组织反应，但染色通常仅限于溃疡区域。这些研究扩展了最近的观察结果，即急性坏死性溃疡性牙龈炎患者牙菌斑中存在病原体相关螺旋体，血清免疫球蛋白G与T. pallidum亚种，提示病原体相关螺旋体可能与某些牙周疾病的发病机制有关。
The purpose of this investigation was to determine whether monoclonal antibodies against pathogen-restricted antigens of Treponema pallidum subsp. pallidum could be used as probes for spirochetes in diseased gingival tissue from subjects with acute necrotizing ulcerative gingivitis. A biotin-streptavidin system was used to identify spirochetes bound by monoclonal antibodies in cryostat sections of tissue. Twelve of 16 tissue samples from diseased sites, but none of 8 tissue specimens from healthy sites, reacted with pathogen-restricted antibodies. Organisms were found in intact epithelium and connective tissues adjacent to ulcers. Staining intensity was often high in perivascular locations and around vesicular spaces. Monoclonal antibodies to Bacteroides gingivalis and Treponema denticola were each reactive with diseased gingival tissues, but staining was usually restricted to ulcerated areas. These studies extend recent observations that showed that subjects with acute necrotizing ulcerative gingivitis had both pathogen-related spirochetes in dental plaque and serum immunoglobulin G to pathogen-restricted antigens on T. pallidum subspecies, suggesting that pathogen-related spirochetes may be associated with the pathogenesis of certain periodontal diseases.