Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development:: possible interaction with peroxidases
Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development:: possible interaction with peroxidases
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DOI：
10.1111/j.1469-8137.2007.01995.x
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发表时间：
2007-01-01
期刊：
影响因子：
9.4
通讯作者：
Penel, Claude
中科院分区：
文献类型：
--
作者：
Dunand, Christophe;Crevecoeur, Michele;Penel, Claude
在拟南芥根尖端中确定了超氧化物（O-2（中心点））和过氧化氢（H2O2）的分布，两种参与根生长和分化的活性氧（ROS）。我们研究了改变这些ROS对根发育水平以及可能与过氧化物酶相互作用的影响。H2O2使用羟基苯基荧光素（HPF）检测到共聚焦激光扫描显微镜检测。分别使用氮气四唑（NBT）和O-二氨酸氨酸评估了O-2（中心点）积累和过氧化物酶分布。在ROS清除后，还对根长度和根长的长度和密度进行了定量。O-2（中心点）主要位于细胞伸长区域的凋亡中，而H2O2在分化区和形成中根毛的细胞壁中积聚。降低O-2（中心点）浓度的处理可降低根部伸长和根部头发的形成，而清除H2O2促进了根部伸长并抑制了根部头发的形成。结果允许确定O-2（中心点 - ）和H2O2在根生长和发育中的各自作用。讨论了它们在根尖端内的不同积累位点的后果，尤其是与过氧化物酶有关。
The respective distribution of superoxide (O-2(center dot-)) and hydrogen peroxide (H2O2), two reactive oxygen species (ROS) involved in root growth and differentiation, was determined within the Arabidopsis root tip. We investigated the effect of changing the levels of these ROS on root development and the possible interactions with peroxidases.H2O2 was detected by confocal laser-scanning microscopy using hydroxyphenyl fluorescein (HPF). Both O-2(center dot-) accumulation and peroxidase distribution were assessed by light microscopy, using nitroblue tetrazolium (NBT) and o-dianisidine, respectively. Root length and root hair length and density were also quantified following ROS scavenging.O-2(center dot-) was predominantly located in the apoplast of cell elongation zone, whereas H2O2 accumulated in the differentiation zone and the cell wall of root hairs in formation. Treatments that decrease O-2(center dot-) concentration reduced root elongation and root hair formation, while scavenging H2O2 promoted root elongation and suppressed root hair formation.The results allow to precise the respective role of O-2(center dot-) and H2O2 in root growth and development. The consequences of their distinct accumulation sites within the root tip are discussed, especially in relation to peroxidases.