Analysis of gene expression in cyclooxygenase-2-overexpressed human osteosarcoma cell lines.
Analysis of gene expression in cyclooxygenase-2-overexpressed human osteosarcoma cell lines.
复制标题
DOI：
10.5808/gi.2014.12.4.247
复制
发表时间：
2014-12
影响因子：
--
通讯作者：
Kim JI
中科院分区：
文献类型：
--
作者：
Han JA;Kim JY;Kim JI
骨肉瘤是最常见的原发性骨肿瘤，通常影响年轻人。虽然骨肉瘤的病因在很大程度上是未知的，但最近的研究表明，环氧合酶-2（考克斯-2）在骨肉瘤细胞的增殖、迁移和侵袭中起关键作用。为了了解考克斯-2在骨肉瘤发病机制中的作用机制，我们比较了三种稳定的考克斯-2过表达细胞系和三种来自U2 OS人骨肉瘤细胞的对照细胞系之间的基因表达模式。数据显示，在考克斯-2过表达的细胞系中，56个基因上调，而20个基因下调，平均倍数变化> 1.5。在上调的基因中，COL 1A 1、COL 5A 2、FBN 1、HOXD 10、RUNX 2和TRAPPC 2参与骨和骨骼系统发育，而DDR2、RAC 2、RUNX 2和TSPAN 31参与细胞增殖的正调控。在下调的基因中，HIST 1H 1D、HIST 1H 2AI、HIST 1H 3 H和HIST 1H 4C参与核小体组装和DNA包装。这些结果为阐明考克斯-2介导的骨肉瘤恶性表型的分子机制提供了有用的信息。
Osteosarcoma is the most common primary bone tumor, generally affecting young people. While the etiology of osteosarcoma has been largely unknown, recent studies have suggested that cyclooxygenase-2 (COX-2) plays a critical role in the proliferation, migration, and invasion of osteosarcoma cells. To understand the mechanism of action of COX-2 in the pathogenesis of osteosarcoma, we compared gene expression patterns between three stable COX-2-overexpressing cell lines and three control cell lines derived from U2OS human osteosarcoma cells. The data showed that 56 genes were upregulated, whereas 20 genes were downregulated, in COX-2-overexpressed cell lines, with an average fold-change > 1.5. Among the upregulated genes, COL1A1, COL5A2, FBN1, HOXD10, RUNX2, and TRAPPC2are involved in bone and skeletal system development, while DDR2, RAC2, RUNX2, and TSPAN31are involved in the positive regulation of cell proliferation. Among the downregulated genes, HIST1H1D, HIST1H2AI, HIST1H3H, and HIST1H4C are involved in nucleosome assembly and DNA packaging. These results may provide useful information to elucidate the molecular mechanism of the COX-2-mediated malignant phenotype in osteosarcoma.