ISOLATED PANCREATIC ISLET ALLOGRAFTS IN RATS RENDERED IMMUNOLOGICALLY UNRESPONSIVE TO RENAL ALLOGRAFTS: The Effect of the Site of Transplantation
ISOLATED PANCREATIC ISLET ALLOGRAFTS IN RATS RENDERED IMMUNOLOGICALLY UNRESPONSIVE TO RENAL ALLOGRAFTS: The Effect of the Site of Transplantation
复制标题
大鼠离体胰岛同种异体移植物对肾同种异体移植物免疫无反应：移植部位的影响
作者：
W. Derek;R. Gray;H. Reece;B. Fairbrother;P. McShane;P. Morris
20只DA（RT-1*）大鼠经环孢素处理14天后，建立了长期存活的刘易斯（RT-11）肾移植（LS-LEW）模型。所有患者在移植后100天使用链脲佐菌素使其患糖尿病; 7例LS-LEW未接受治疗，所有患者仍患糖尿病; 5例LS-LEW在肾包膜下给予刘易斯胰岛，未进一步免疫抑制。在4只大鼠中观察到移植物存活延长（>100天）。其中5例经门静脉注入刘易斯胰岛。4只大鼠移植物存活时间延长。在3个LS-LEW中，在肾包膜下给予第三方BN胰岛;这些胰岛在不到9天内被排斥。相比之下，将刘易斯胰岛移植到未治疗的糖尿病DA大鼠的肾包膜下或门静脉中，分别存活平均8.3天和4天。在一项单独的实验中，通过环孢霉素处理在7只PVG大鼠（LS-PVG）中诱导了长期存活的刘易斯肾移植物。移植后100天使这些动物患糖尿病，然后在移植肾的肾包膜下给予刘易斯胰岛。在6只大鼠中观察到胰岛移植物存活时间延长，5只糖尿病PVG大鼠在肾包膜下给予刘易斯胰岛，8天内胰岛排斥。因此，一旦受体大鼠在环孢霉素治疗的影响下接受了肾同种异体移植物，它将永久接受与肾相同品系的胰岛同种异体移植物。该效应适用于测试的两种菌株组合，不受胰岛植入部位的影响，并且对与肾同种异体移植物相同菌株的胰岛具有特异性。
Long-surviving Lewis (RT-11) renal allografts (LS-LEW) were induced in 20 DA (RT-1*) rats by 14-day treatment with cyclosporine. All were made diabetic 100 days after transplantation using streptozotocin; 7 LS-LEW were untreated and all remained diabetic; 5 LS-LEW were given Lewis islets beneath the kindney capsule without further immunosuppression. Prolonged graft survival (>100 days) was seen in 4 rats. Lewis islets were given into the portal vein in 5 LS-LEW. Prolonged graft survival was seen in 4 rats. Third-party BN islets were given beneath the kidney capsule in 3 LS-LEW; these islets were rejected in less than 9 days. In contrast Lewis islets transplanted into untreated diabetic DA rats beneath the renal capsule or into the portal vein survived for a mean of 8.3 days and 4 days, respectively. In a separate experiment long-surviving Lewis renal allografts were induced in 7 PVG rats (LS-PVG) by cyclosporine treatment. These animals were made diabetic 100 days after transplantation and then were given Lewis islets under the renal capsule of the transplant kidney. Prolonged islet graft survival was seen in 6 rats, and 5 diabetic PVG rats given Lewis islets beneath the renal capsule rejected the islets within 8 days. Thus, once a recipient rat has accepted a renal allograft under the influence of cyclosporine treatment, it will accept permanently an islet allograft of the same strain as the kidney. This effect applies to both strain combinations tested, is not influenced by the site of islet implantation, and is specific for islets of the same strain as the renal allograft.