Mutation of FVS1, encoding a protein with a sterile alpha motif domain, affects asexual reproduction in the fungal plant pathogen Fusarium oxysporum
Mutation of FVS1, encoding a protein with a sterile alpha motif domain, affects asexual reproduction in the fungal plant pathogen Fusarium oxysporum
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DOI：
10.1111/1574-6968.12356
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发表时间：
2014-02-01
影响因子：
2.1
通讯作者：
Tsuge, Takashi
中科院分区：
文献类型：
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作者：
Iida, Yuichiro;Fujiwara, Kazuki;Tsuge, Takashi
尖孢镰刀菌产生三种无性孢子：小孢子、大分生孢子和厚壁孢子。我们先前分析了尖孢霉营养生长和分生过程中的表达序列标签，发现42个基因在分生过程中与营养生长相比明显上调。其中一个基因FVS1编码一个带有不育α基序(SAM)结构域的蛋白质，该结构域在参与转录或转录后调控和信号转导的蛋白质-蛋白质相互作用中发挥作用。在这里，我们从甜瓜枯萎病病原菌F.oxporum f.sp.中获得了FVS1-破坏突变体。甜瓜。虽然突变体产生了形态正常的三种无性孢子，但它们形成的小孢子和大分生孢子明显少于野生型。突变体在形成小分生孢子和大分生孢子所需的分生孢子发生细胞、分生孢子梗和菌丝的发育方面似乎存在缺陷。相反，在突变体中厚垣孢子的形成被显著地促进。突变体在培养基上的生长速率略有下降，表明FVS1也参与了营养生长，但不是必需的。我们还观察到，Fvs1的突变导致了分生孢子萌发和致病力的缺陷，表明Fvs1在尖孢镰刀菌中具有多效性。
Fusarium oxysporum produces three kinds of asexual spores: microconidia, macroconidia and chlamydospores. We previously analysed expressed sequence tags during vegetative growth and conidiation in F.oxysporum and found 42 genes that were markedly upregulated during conidiation compared to vegetative growth. One of the genes, FVS1, encodes a protein with a sterile alpha motif (SAM) domain, which functions in protein-protein interactions that are involved in transcriptional or post-transcriptional regulation and signal transduction. Here, we made FVS1-disrupted mutants from the melon wilt pathogen F.oxysporum f. sp. melonis. Although the mutants produced all three kinds of asexual spores with normal morphology, they formed markedly fewer microconidia and macroconidia than the wild type. The mutants appeared to have a defect in the development of the conidiogenesis cells, conidiophores and phialides, required for the formation of microconidia and macroconidia. In contrast, chlamydospore formation was dramatically promoted in the mutants. The growth rates of the mutants on media were slightly reduced, indicating that FVS1 is also involved in, but not essential for, vegetative growth. We also observed that mutation of FVS1 caused defects in conidial germination and virulence, suggesting that the Fvs1 has pleiotropic functions in F.oxysporum.