Assessment of the clinical significance of serum matrix metalloproteinases MMP-2 and MMP-9 in patients with various chronic liver diseases and hepatocellular carcinoma
Assessment of the clinical significance of serum matrix metalloproteinases MMP-2 and MMP-9 in patients with various chronic liver diseases and hepatocellular carcinoma
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DOI：
10.1055/s-0037-1613578
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发表时间：
2003-04-01
影响因子：
6.7
通讯作者：
Verspaget, HW
中科院分区：
文献类型：
--
作者：
Kuyvenhoven, JP;van Hoek, B;Verspaget, HW
基质金属蛋白酶（MMPs）具有降解基底膜的能力，因此可能在肝纤维化和癌变的细胞外基质转换中起重要作用。采用酶联免疫吸附法（ELISA）检测了91例慢性肝病患者（其中25例为肝细胞癌（HCC））和60例正常对照者血清中MMP-2和MMP-9的水平，发现慢性肝病患者血清中MMP-2水平明显高于正常对照者，且随Child-Pugh分级升高而升高。MMP-2与肝功能（胆红素、白蛋白和凝血酶原时间）之间存在显著相关性，MMP-9与这些参数之间存在强烈的相反相关性。MMP-2在HCC患者中的水平显著高于对照组，但与无此恶性肿瘤的慢性肝病患者相当。血清MMP-2和MMP-9在较小程度上与肝脏疾病的严重程度相关，并可能反映细胞外基质重塑的变化。由于慢性肝病伴或不伴HCC患者中存在相当大的重叠，MMP-2和MMP-9不能用作HCC的诊断标志物。
Matrix metalloproteinases (MMPs) have the ability to degrade basement membranes and may thus play an important role in extracellular matrix turnover in liver fibrosis and carcinogenesis. Serum levels of MMPs have been suggested as diagnostic markers in these processes.We measured serum MMP-2 and MMP-9 by ELISA in 91 patients with chronic liver disease, including 25 patients with hepatocellular carcinoma (HCC), and in 60 controls.MMP-2 was significantly higher in patients with chronic liver disease compared to controls, and increased with Child-Pugh class. There was a significant correlation between MMP-2 and liver function (bilirubin, albumin, and prothrombin time), and a strong opposite correlation between MMP-9 and these parameters. MMP-2 levels in patients with HCC were significantly higher than in controls, but comparable to patients with chronic liver disease without this malignancy. MMP-9 yielded no significant differences between patients with or without HCC and controls.Serum MMP-2 and to a lesser extent MMP-9 correlate with the severity of liver disease and may reflect changes in extracellular matrix remodeling. Due to a considerable overlap in patients with chronic liver disease with or without HCC, MMP-2 and MMP-9 can not be used as a diagnostic marker for HCC.