Soil bacterial diversity changes in different broomcorn millet intercropping systems
Soil bacterial diversity changes in different broomcorn millet intercropping systems
复制标题
不同黍间作系统土壤细菌多样性变化
DOI：
10.1002/jobm.201700133
复制
发表时间：
2017-12-01
影响因子：
3.1
通讯作者：
Qiao, Zhijun
中科院分区：
文献类型：
--
作者：
Cao, Xiaoning;Liu, Sichen;Qiao, Zhijun
生长在土壤中的植物和它们直接接触的各种微生物已经适应了利用它们的密切联系实现互利。各种间作制度已被用于控制植物病害和提高田间生产力。虽然高通量测序方法为土壤细菌群落提供了新的见解，但目前对高粱与不同豆科植物间作的了解有限。在这项研究中，通过深度测序获得了单作和间作系统的不同细菌群落的特征。共有4684个可操作分类单元被分类到物种水平，具有良好的采样深度和测序覆盖率。放线杆菌、类杆菌、变形杆菌、酸性细菌和双生单胞菌在不同生长阶段的丰度不同，并与间作植物的生长有关。根据多样性分析，糖霉属、气微菌属、粘着杆菌属和链霉菌属是优势属。此外，我们还根据细菌OTUS的存在预测了功能基因的组成。功能结果表明，所有样品的膜转运和营养物质代谢都非常丰富，尽管丰度在不同的生长阶段有所不同，这表明这些途径可能受到细菌群落优势类的影响。在高粱与不同豆科植物间作过程中观察到的动态变化表明，土壤细菌群落结构呈现出作物物种特有的格局。此外，不同间作系统的谷子农艺性状数据与功能结果一致，表明土壤细菌群落可能影响了谷子农艺性状。
Plants growing in soil and the diverse microorganisms with which they are in direct contact have adapted to exploit their close association for mutual benefit. Various intercropping systems have been used to control plant disease and improve productivity in fields. Although high-throughput sequencing approaches have provided new insights into the soil bacterial community, current knowledge of intercropping of broomcorn millet with different leguminous plants is limited. In this study, characterization of different bacterial communities of monoculture and intercropping systems was achieved by deep sequencing. A total of 4684 operational taxonomic units were classified to the species level with good sampling depth and sequencing coverage. The abundance of Actinobacteria, Bacteroidetes, Proteobacteria, Acidobacteria, and Gemmatimonadetes varied at different growth stages and was related to growth of the intercropped plant. According to diversity analyses, Glycomyces, Aeromicrobium, Adhaeribacter, and Streptomyces were the dominant genera. In addition, we predicted functional gene composition based on bacterial OTUs present. Functional results showed that membrane transport and nutrient metabolism was highly abundant in all samples, although abundance varied at different growth stages, which indicated these pathways might be affected by the dominant categories of bacterial community. The dynamic changes observed during intercropping of broomcorn millet with different leguminous plants suggest that soil bacterial community structure exhibits a crop species-specific pattern. Further, agronomic trait data from different broomcorn millet intercropping systems were consistent with functional results and suggest that agronomic traits may be influenced by soil bacterial communities.