Stereo vision in spatial-light-modulator-based microscopy.
Stereo vision in spatial-light-modulator-based microscopy.
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DOI：
10.1364/ol.37.002238
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发表时间：
2012-06
期刊：
影响因子：
3.6
通讯作者：
M. Hasler;T. Haist;W. Osten
中科院分区：
文献类型：
--
作者：
M. Hasler;T. Haist;W. Osten
我们提出了一种实现立体显微镜的技术。我们采用一个基于空间光调制器的显微镜，在一次拍摄中记录两个不同角度下的图像。我们还调查了动态像差校正的可能性。研究发现，由于调制器采用的是商用硅基液晶，像差校正是不可避免的。此外，相位物体和高反射试样的成像进行了实验研究。对于一些标本，观察到记录强度的反转，这导致在观看立体对时出现问题。我们解释了这种效果的起源，并表明，一个合理的可视化微观三维物体可以通过简单的图像反转。
We propose a technique for realizing stereoscopic microscopy. We employ a spatial-light-modulator-based microscope to record two images under different angles in one shot. We additionally investigate the possibilities of dynamic aberration correction. It is found that aberration correction is unavoidable because of the employed commercial liquid crystal on a silicon modulator. Also, imaging of phase objects and highly reflective specimens is experimentally investigated. For some of the specimens, an inversion of the recorded intensity is observed, which leads to problems when viewing the stereo pairs. We explain the origin of this effect and show that a reasonable visualization of microscopic three-dimensional objects can be achieved by simple image inversion.