Immunization with parasite-derived apical membrane antigen 1 or passive immunization with a specific monoclonal antibody protects BALB/c mice against lethal Plasmodium yoelii yoelii YM blood-stage infection
Immunization with parasite-derived apical membrane antigen 1 or passive immunization with a specific monoclonal antibody protects BALB/c mice against lethal Plasmodium yoelii yoelii YM blood-stage infection
复制标题
DOI：
10.1128/iai.68.5.2899-2906.2000
复制
发表时间：
2000-05-01
影响因子：
3.1
通讯作者：
Holder, AA
中科院分区：
文献类型：
--
作者：
Narum, DL;Ogun, SA;Holder, AA
我们从感染了啮齿动物疟原虫yoelii yoelii YM的红细胞提取物中纯化了尖裂子抗原1 （AMA-1）。当用于免疫小鼠时，这种蛋白质诱导了一种强烈的保护性反应，以抵抗寄生虫的攻击。制备了针对yoelii yoelii AMA-1的单克隆抗体，其中一种单克隆抗体在被动免疫条件下对该寄生虫具有很好的抑制作用。第二个蛋白似乎位于根尖状细胞器中，并与AMA-1相关。
We have purified apical merozoite antigen 1 (AMA-1) from extracts of red blood cells infected with the rodent malaria parasite Plasmodium yoelii yoelii YM. When used to immunize mice, the protein induced a strong protective response against a challenge with the parasite. Monoclonal antibodies specific for P. yoelii yoelii AMA-1 were prepared, and one was very effective against the parasite on passive immunization. A second protein that appears to be located in the apical rhoptry organelles and associated with AMA-1 was identified.