Effects of the Cowpea chlorotic mottle bromovirus β-hexamer structure on virion assembly
Effects of the Cowpea chlorotic mottle bromovirus β-hexamer structure on virion assembly
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DOI：
10.1016/s0042-6822(02)00054-5
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发表时间：
2003-02-15
期刊：
影响因子：
3.7
通讯作者：
Young, MJ
中科院分区：
文献类型：
--
作者：
Willits, D;Zhao, X;Young, MJ
豇豆褪绿斑驳病毒（CCMV）的 X 射线晶体结构揭示了一种独特的管状结构，该结构是由组装病毒粒子的每个三重轴上的六个外壳蛋白亚基的 N 末端相互作用形成的。这种结构称为β-六聚体，由六个短β-链组成。 β-六聚体被认为通过稳定六聚壳粒在病毒粒子的组装和稳定性中发挥关键作用（Speir 等人，1995）。利用体外和体内组装测定对β-六聚体结构进行的突变分析表明，这种结构对于体外无核酸的病毒体形成或体内含有RNA的病毒体来说不是必需的。然而，β-六聚体结构确实有助于体外病毒粒子的稳定性并调节体内疾病的表达。这些结果支持通过五聚体中间体进行 CCMV 组装的模型。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
The X-ray crystal structure of Cowpea chlorotic mottle bromovirus (CCMV) revealed a unique tubular structure formed by the interaction of the N-termini from six coat protein subunits at each three-fold axis of the assembled virion. This structure, termed the beta-hexamer, consists of six short beta-strands. The beta-hexamer was postulated to play a critical role in the assembly and stability of the virion by stabilizing hexameric capsomers (Speir et al., 1995). Mutational analyses of the beta-hexamer structure, utilizing both in vitro and in vivo assembly assays, demonstrate that this structure is not required for virion formation devoid of nucleic acids in vitro or for RNA-containing virions in vivo. However, the beta-hexamer structure does contribute to virion stability in vitro and modulates disease expression in vivo. These results support a model for CCMV assembly through pentamer intermediates. (C) 2003 Elsevier Science (USA). All rights reserved.