Effect of leptin treatment on neonatal hypoxic-ischemic brain injury
Effect of leptin treatment on neonatal hypoxic-ischemic brain injury
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DOI：
10.3109/14767058.2011.565834
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发表时间：
2012-02-01
影响因子：
1.8
通讯作者：
Ozkan, Hasan
中科院分区：
文献类型：
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作者：
Kumral, Abdullah;Yesilirmak, Didem Cemile;Ozkan, Hasan
Objective.本研究的目的是探讨瘦素治疗对缺氧缺血性损伤诱导的神经细胞死亡的可能的神经保护和改善作用。实验分为假手术组、瘦素治疗组和溶剂治疗组。缺氧缺血组于生后第7天永久性结扎左侧颈总动脉。手术后2小时，缺氧（92%氮气和8%氧气）2.5小时。注射瘦素治疗（腹膜内; i.第页）缺氧期后立即单次给药。在缺氧缺血性损伤后72 h，评估两个半球的神经细胞死亡、神经元密度和瘦素水平。与缺氧缺血组相比，假手术组两侧大脑半球的瘦素水平均升高。瘦素治疗显著减少了海马CA 1、CA 2、CA 3和两个半球齿状回区域的“凋亡细胞”数量。瘦素治疗显着保存在两个半球的神经元的数量，与车辆处理组相比。我们的结论是，瘦素治疗可以改善缺氧缺血性脑损伤新生大鼠的神经元密度并减少细胞凋亡。
Objective. The aim of this study was to investigate the possible neuroprotective and ameliorating effects of leptin treatment in hypoxic-ischemic injury induced neuronal cell death.Methods. Experimental groups in the study were: sham-operated group, leptin treated hypoxia-ischemia group, and vehicle treated hypoxia-ischemia group. In hypoxia-ischemia group, left common carotid artery was ligated permanently on the seventh postnatal day. Two hours after the procedure, hypoxia (92% nitrogen and 8% oxygen) was applied for 2.5 h. Leptin treatment was injected (intraperitoneally; i. p.) as a single dose immediately after the hypoxia period. Neuronal cell death, neuronal density, and leptin levels were evaluated in both hemispheres 72 h after the hypoxic-ischemic insult.Results. Compared with the hypoxic-ischemia group, the mean leptin levels were higher in the brains of the sham group for both hemispheres. The leptin treatment significantly diminished the number of 'apoptotic cells' in the hippocampal CA1, CA2, CA3, and gyrus dentatus regions in both hemispheres. Leptin treatment significantly preserved the number of neurons in both hemispheres, when compared with the vehicle treated group.Conclusion. We conclude that leptin treatment improves neuronal density and decreases apoptosis in the newborn rat with hypoxic-ischemic brain injury.