Human skin organ culture for assessment of chemically induced skin damage.
Human skin organ culture for assessment of chemically induced skin damage.
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DOI：
10.1586/edm.12.24
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发表时间：
2012-06-01
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影响因子：
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通讯作者：
Varani J
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文献类型：
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作者：
Varani J
远离动物模型进行皮肤安全测试是不可避免的。这是一个时间的问题，而不是如果。随着皮肤安全性研究远离传统的动物方法，一些替代技术正在变得可用。器官培养中的人类皮肤就是这样一种技术。器官培养的皮肤有几个特点，使其区别于其他技术。首先，器官培养的皮肤是真实的皮肤。因此，几乎根据定义，它比其他替代模型更接近完整皮肤。器官培养是一种易于使用且相对便宜的临床前安全性评估方法。虽然器官培养不太可能取代高通量酶测定或单层培养/皮肤等效培养用于初始化合物评估，但当待评价的化合物列表较少且较简单的模型缩小了剂量范围时，器官培养应可派上用场。器官培养的皮肤也为机制研究提供了平台。
The move away from animal models for skin safety testing is inevitable. It is a question of when, not if. As skin safety studies move away from traditional animal-based approaches, a number of replacement technologies are becoming available. Human skin in organ culture is one such technology. Organ-cultured skin has several features that distinguish it from other technologies. First and foremost, organ-cultured skin is real skin. Almost by definition, therefore, it approximates the intact skin better than other alternative models. Organ culture is an easy-to-use and relatively inexpensive approach to preclinical safety assessment. Although organ culture is not likely to replace high-throughput enzyme assays or monolayer culture/skin equivalent cultures for initial compound assessment, organ culture should find use when the list of compounds to be evaluated is small and when simpler models have narrowed the dose range. Organ-cultured skin also provides a platform for mechanistic studies.