Biosynthesis of d-lactic acid from lignocellulosic biomass
Biosynthesis of d-lactic acid from lignocellulosic biomass
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DOI：
10.1007/s10529-018-2588-2
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发表时间：
2018-06
影响因子：
2.7
通讯作者：
Yixing Zhang;Makoto Yoshida;P. Vadlani
中科院分区：
文献类型：
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作者：
Yixing Zhang;Makoto Yoshida;P. Vadlani
d-乳酸是一种用途广泛的重要工业化学品，可用于合成耐热聚乳酸。d-乳酸的生物合成可以通过多种微生物实现，包括乳酸菌、酵母菌和真菌；然而，最终产物收率、光学纯度以及葡萄糖和木糖的利用率都受到限制。因此，工程微生物系统是必不可少的，以达到高滴度，生产力和糖的完全利用。在此，我们批判性地评估了有前途的野生型微生物，以及转基因微生物，以生产对构象纯乳酸，特别是从可再生的木质纤维素生物质。此外，将讨论创新的生物反应器操作，代谢通量分析和靶向微生物乳酸合成的最新基因工程方法。
d-lactic acid is a versatile and important industrial chemical that can be applied in the synthesis of thermal-resistant poly-lactic acid. Biosynthesis ofd-lactic acid can be achieved by a variety of microorganisms, including lactic acid bacteria, yeast, and fungi; however, the final product yield, optical purity, and the utilization of both glucose and xylose are restricted. Consequently, engineered microbial systems are essential to attain high titer, productivity, and complete utilization of sugars. Herein, we critically evaluate the promising wild-type microorganisms, as well as genetically modified microorganisms to produce enantiomerically pured-lactic acid, particularly from renewable lignocellulosic biomass. In addition, innovative bioreactor operation, metabolic flux analysis, and recent genetic engineering methods for targeted microbiald-lactic acid synthesis will be discussed.