Label-free optical imaging in developmental biology [Invited].
Label-free optical imaging in developmental biology [Invited].
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DOI：
10.1364/boe.381359
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发表时间：
2020-03
影响因子：
3.4
通讯作者：
Shang Wang;I. Larina;K. Larin
中科院分区：
文献类型：
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作者：
Shang Wang;I. Larina;K. Larin
光学成像在发育生物学中的应用是生物医学光学领域的一个前沿课题。光学分辨率和成像深度允许在亚细胞，细胞和整个生物体水平上研究生长中的胚胎，而胚胎过程的复杂性和多样性设置了多种挑战，刺激了各种活动态胚胎成像方法的发展。在其他光学方法中，无标记光学技术吸引了越来越多的兴趣，因为它们允许在不应用外源标记或荧光报告的情况下研究发育机制。在过去的十年中，用于研究动物模型中胚胎发育的无标记光学成像技术的发展得到了推动，这揭示了有关早期发育的新信息，并为研究创造了新的领域。在这里，我们回顾了无标记光学胚胎成像的最新进展，讨论了具体的应用，并评论了未来的发展在光子学，工程学和发育生物学的接口。
Application of optical imaging in developmental biology marks an exciting frontier in biomedical optics. Optical resolution and imaging depth allow for investigation of growing embryos at subcellular, cellular, and whole organism levels, while the complexity and variety of embryonic processes set multiple challenges stimulating the development of various live dynamic embryonic imaging approaches. Among other optical methods, label-free optical techniques attract an increasing interest as they allow investigation of developmental mechanisms without application of exogenous markers or fluorescent reporters. There has been a boost in development of label-free optical imaging techniques for studying embryonic development in animal models over the last decade, which revealed new information about early development and created new areas for investigation. Here, we review the recent progress in label-free optical embryonic imaging, discuss specific applications, and comment on future developments at the interface of photonics, engineering, and developmental biology.