Inducible aluminum resistance of Acidiphilium cryptum and aluminum tolerance of other acidophilic bacteria
Inducible aluminum resistance of Acidiphilium cryptum and aluminum tolerance of other acidophilic bacteria
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DOI：
10.1007/s00203-002-0482-7
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发表时间：
2002-09
影响因子：
2.8
通讯作者：
J. Fischer;A. Quentmeier;Sven Gansel;V. Sabadoš;C. Friedrich
中科院分区：
文献类型：
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作者：
J. Fischer;A. Quentmeier;Sven Gansel;V. Sabadoš;C. Friedrich
铝离子在酸性环境中高度可溶。研究了铝离子对异养、兼性和专性化能无机自养嗜酸菌的毒性，结果表明，隐嗜酸菌在含300 mM硫酸铝[Al_2（SO_4）_3]的葡萄糖-无机培养基（pH 3）中生长，滞后期约为120 h，倍增时间为7.6 h，而不含铝的生长为5.2 h。用1 mM的Al_2（SO_4）_3预培养并转移到含300 mM Al_2（SO_4）_3的培养基中，将滞后期从120小时缩短到60小时，当A.用50 mM Al 2（SO 4）3预培养，表明铝诱导的抗性。Fe ~（3+）和二价阳离子均不诱导耐铝性。在暴露于A. 300 mM Al 2（SO 4）3处理后，SDS-PAGE检测蛋白质谱发生显著变化。当用50-200 mM硫酸铝培养其他嗜酸菌时，没有观察到滞后期，而生长速率和细胞产率显著降低。在Acidobacterium capsulatum、Acidiphilium acidophilum、Acidithiobacillus ferrooxidans和Acidithiobacillus thiooxidans中观察到这种生长反应。用铝离子预培养这些菌株并没有改变铝引起的生长反应。A.在300 mM Al 2（SO 4）3培养基中培养的菌株的铝含量为0.44 μg Al/mg细胞干重，而在50 mM Al 2（SO 4）3培养基中培养的菌株的铝含量为0.30 ~ 3.47 μg Al/mg细胞干重。
Aluminum ions are highly soluble in acidic environments. Toxicity of aluminum ions for heterotrophic, facultatively and obligately chemolithoautotrophic acidophilic bacteria was examined.Acidiphilium cryptumgrew in glucose-mineral medium, pH 3, containing 300 mM aluminum sulfate [Al2(SO4)3] after a lag phase of about 120 h with a doubling time of 7.6 h, as compared to 5.2 h of growth without aluminum. Precultivation with 1 mM Al2(SO4)3and transfer to a medium with 300 mM Al2(SO4)3reduced the lag phase from 120 to 60 h, and immediate growth was observed whenA. cryptumwas precultivated with 50 mM Al2(SO4)3, suggesting an aluminum-induced resistance. Aluminum resistance was not induced by Fe3+ions and divalent cations. Upon exposure ofA. cryptumto 300 mM Al2(SO4)3, the protein profile changed significantly as determined by SDS-PAGE. When other acidophiles were cultivated with 50–200 mM aluminum sulfate, no lag phase was observed while the growth rates and the cellular yields were significantly reduced. This growth response was observed withAcidobacterium capsulatum,Acidiphilium acidophilum,Acidithiobacillus ferrooxidans, andAcidithiobacillus thiooxidans. Precultivation of these strains with aluminum ions did not alter the growth response caused by aluminum. The content ofA. cryptumcultivated with 300 mM Al2(SO4)3was 0.44 µg Al/mg cell dry weight, while that of the other strains cultivated with 50 mM Al2(SO4)3ranged from 0.30 to 3.47 µg Al/mg cell dry weight.