Prostaglandin E2 synthesis in the inner medullary collecting duct of the rat: implications for vasopressin-dependent cyclic AMP formation.
Prostaglandin E2 synthesis in the inner medullary collecting duct of the rat: implications for vasopressin-dependent cyclic AMP formation.
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大鼠内髓集合管中前列腺素 E2 的合成：对加压素依赖性环 AMP 形成的影响。
DOI：
10.1002/jcp.1041290109
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发表时间：
1986
影响因子：
5.6
通讯作者：
Jackson,BA
中科院分区：
文献类型：
--
作者：
Jackson,BA
在胶原酶消化的大鼠肾脏微解剖的乳头状收集管（PCD）中，研究了渗透压对前列腺素E2（PGE2）生物合成能力的影响，以及内源性PGE2合成与抗利尿激素（AVP）依赖性环AMP生成之间的相互作用。用NaCl：尿素（1:2摩尔比）增加介质渗透压，PCD中pge2的合成逐渐增加，达到1,500 mOsm。添加NaCl：尿素或单独添加NaCI对刺激PCD中pge2的生物合成能力同样有效。相比之下，单独添加尿素对pge2合成的刺激作用要小得多。在300 mOsm培养基中，萘普生（10−5M）抑制内源性pge2合成，抑制了PCD中AVP依赖性cAMP的形成，但在1500 mOsm培养基中没有影响。当在300 mOsm培养基中孵育时，2.5×10−5M pge2也抑制了PCD中AVP依赖性cAMP的形成。目前的研究表明PCD是一个活性pge2合成的位点，受渗透压调节。我们的研究结果不支持内源性pge2通过抑制AVP依赖性cAMP的形成来拮抗血管加压素作用的概念。
The effects of osmolality on prostaglandin E2(PGE2) biosynthetic capacity and the interaction between endogenous PGE2synthesis and vasopressin (AVP)‐dependent cyclic AMP generation were examined in papillary collecting ducts (PCD) microdissected from collagenase‐digested rat kidneys. Increasing medium osmolality with NaCl:urea (1:2 molar ratio) progressively increased PGE2synthesis in PCD up to 1,500 mOsm. Addition of NaCl:urea or NaCI alone were equally effective in stimulating PGE2biosynthetic capacity in PCD. In contrast, addition of urea alone had a much smaller stimulatory effect on PGE2synthesis. Inhibition of endogenous PGE2synthesis with naproxen (10−5M) suppressed AVP‐dependent cAMP formation in PCD when incubated in 300 mOsm medium but had no effect when incubated in 1,500 mOsm medium. Addition of 2.5×10−5M PGE2also suppressed AVP‐dependent cAMP formation in PCD only when incubated in 300 mOsm medium. The present study suggests that the PCD is a site of active PGE2synthesis that is modulated by osmolality. Our results do not support the concept that endogenous PGE2antagonizes vasopressin action via inhibition of AVP‐dependent cAMP formation.