A COMPARATIVE EPR INVESTIGATION OF THE MULTICOPPER PROTEINS NITROUS-OXIDE REDUCTASE AND CYTOCHROME-C-OXIDASE
A COMPARATIVE EPR INVESTIGATION OF THE MULTICOPPER PROTEINS NITROUS-OXIDE REDUCTASE AND CYTOCHROME-C-OXIDASE
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DOI：
10.1111/j.1432-1033.1992.tb17360.x
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发表时间：
1992-11-01
期刊：
影响因子：
--
通讯作者：
KRONECK, PMH
中科院分区：
文献类型：
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作者：
ANTHOLINE, WE;KASTRAU, DHW;KRONECK, PMH
通过 EPR 光谱在 2.4 - 35 GHz 微波频率下研究多铜蛋白、一氧化二氮还原酶 (N2OR) 和细胞色素 c 氧化酶 (COX)。我们的结果支持 COX 和 N2OR 中的 Cu-Cu 相互作用。在 2.7 GHz 频谱中至少有 10 条谱线、在 4.6 GHz 频谱中至少有 12 条谱线、在 9.2 GHz 频谱中至少有 14 条谱线被解析为 N2OR。 COX 解析了 8 条 2.7 GHz 铜线、大约 9 条 4.6 GHz 铜线和大约 6 条 9.2 GHz 铜线。 EPR 光谱的模拟与多线光谱的大多数共振一致，包括建议三个七线图案重叠的光谱中心区域。这些模拟表明，混合价 [Cu(1.5) ... Cu(1.5)]、S = 1/2 位点中的 Cu-Cu 相互作用与 N2OR 和 COX 观察到的不寻常光谱特征一致（如果不能证明的话）。
The multicopper proteins, nitrous-oxide reductase (N2OR) and cytochrome c oxidase (COX), were investigated by EPR spectroscopy at microwave frequencies 2.4 - 35 GHz. Our results support a Cu-Cu interaction in COX and N2OR. At least 10 lines in the 2.7-GHz, 12 lines in the 4.6-GHz and 14 lines in the 9.2 GHz spectra were resolved for N2OR. Eight copper lines at 2.7 GHz, about nine lines at 4.6 GHz and about six lines at 9.2 GHz were resolved for COX. Simulations of the EPR spectra were consistent with most of the resonances of the multiline spectra, including regions in the center of the spectra where overlap of the three seven-line patterns is proposed. These simulations indicated that Cu-Cu interaction, in a mixed-valence [Cu(1.5) ... Cu(1.5)], S = 1/2 site is consistent with, if not proof of, the unusual spectral features observed for N2OR and COX.