Cytoplasmic inclusion bodies are detected by synthetic antibody in ciliated bronchial epithelium during acute Kawasaki disease
Cytoplasmic inclusion bodies are detected by synthetic antibody in ciliated bronchial epithelium during acute Kawasaki disease
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DOI：
10.1086/497171
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发表时间：
2005-11-15
影响因子：
6.4
通讯作者：
Orenstein, JM
中科院分区：
文献类型：
--
作者：
Rowley, AH;Baker, SC;Orenstein, JM
背景资料。在发达国家，川崎病(KD)是儿童获得性心脏病最常见的原因。可能是感染性病原学，但尚未确定。我们之前已经报道过，寡克隆免疫球蛋白A浆细胞渗透到急性KD组织中，合成的KD抗体在急性KD纤毛支气管壁上皮细胞中检测到一种独特的球状抗原。为了进一步鉴定急性KD支气管中的抗原，我们用光学显微镜(LM)和透射电子显微镜(TEM)检查了石蜡包埋的纤毛支气管壁上皮。免疫组织化学(IHC)观察到的球体为包涵体，苏木精-伊红和核酸染色，亚甲蓝/天青II/碱性品红三色染色的塑料切片上可见包涵体，表明蛋白质和核酸都存在。光镜下可见4例急性KD患者纤毛上皮内均一的电子致密包涵体，直径可达1.4微米。对照组的支气管上皮或无纤毛的细胞中未见包涵体。用合成的KD抗体在急性KD纤毛上皮中检测到的抗原存在于细胞质内，与病毒蛋白和相关核酸的聚集体一致，可能来源于KD的病原体。
Background. In developed nations, Kawasaki disease (KD) is the most common cause of acquired heart disease in children. An infectious etiology is likely but has not yet been identified. We have previously reported that oligoclonal immunoglobulin A plasma cells infiltrate acute KD tissues and that synthetic KD antibodies detect a distinctive spheroidal antigen in acute KD ciliated bronchial epithelium.Methods. To further characterize the antigen in acute KD bronchi, we examined paraffin-embedded ciliated bronchial epithelium using light microscopy (LM) and transmission electron microscopy (TEM).Results. The spheroids observed by immunohistochemistry (IHC) are visualized as inclusion bodies with hematoxylin-eosin and nucleic acid stains and in methylene blue/azure II/basic fuchsin trichrome - stained plastic sections, suggesting the presence of both protein and nucleic acid. The structures visualized by LM correspond to homogeneous electron-dense perinuclear inclusion bodies ( up to 1.4 microns in diameter) in ciliated bronchial epithelium from 4 patients with acute KD examined by TEM. Inclusion bodies were not present in control bronchial epithelium or in nonciliated cells.Conclusions. The antigen detected in acute KD ciliated bronchial epithelium by IHC with synthetic KD antibodies resides in cytoplasmic inclusion bodies that are consistent with aggregates of viral proteins and associated nucleic acid and may derive from the etiologic agent of KD.