DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN SEMEN - EFFECTS OF DISEASE STAGE AND NUCLEOSIDE THERAPY
DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN SEMEN - EFFECTS OF DISEASE STAGE AND NUCLEOSIDE THERAPY
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DOI：
10.1093/infdis/167.4.798
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发表时间：
1993-04-01
影响因子：
6.4
通讯作者：
MERIGAN, TC
中科院分区：
文献类型：
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作者：
HAMED, KA;WINTERS, MA;MERIGAN, TC
应用聚合酶链反应（PCR）技术，探讨感染临床分期和抗病毒治疗对精液中人类免疫缺陷病毒1型（HIV-1）核酸检测的影响。在36名男性中，29名（81%）的52份精液标本中，45份（87%）检出HIV-1。22例II期或III期受试者中的17例（77%）和14例IV期受试者中的12例（86%）标本呈阳性。精液阳性和阴性受试者的CD 4+淋巴细胞计数无显著差异。此外，6（67%）9未经治疗的男性有阳性标本相比，2 ',3（85%）27人治疗齐多夫定，2'，3 '-双脱氧肌苷，或两者的平均20个月。因此，精液中HIV-1的检测与感染阶段和长期治疗无关。在一项对6名男性进行的核苷治疗开始后8周的半定量分析中，2名男性精浆中的HIV-1 RNA降低。
The effects of clinical stage of infection and antiviral therapy on the detection of human immunodeficiency virus type 1 (HIV-1) nucleic acids in semen were investigated by the polymerase chain reaction. HIV-1 was detected in 45 (87%) of 52 semen specimens from 29 (81%) of 36 men. Seventeen (77%) of 22 stage II or III subjects and 12 (86%) of 14 stage IV subjects had positive specimens. The CD4+ lymphocyte count was not significantly different comparing subjects with positive and negative semen. Moreover, 6 (67%) of 9 untreated men had positive specimens compared with 23 (85%) of 27 men treated with zidovudine, 2',3'-dideoxyinosine, or both for a mean of 20 months. Thus, the detection of HIV-1 in semen was independent of both stage of infection and long-term treatment. In a semiquantitative analysis of 6 men followed for 8 weeks after the start of nucleoside therapy, a decrease in HIV-1 RNA in seminal plasma was demonstrated in 2.