Stages of oocyte development in the pipefish, Syngnathus scovelli
Stages of oocyte development in the pipefish, Syngnathus scovelli
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海龙卵母细胞发育阶段
DOI：
10.1002/jmor.1051970309
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发表时间：
1988
影响因子：
1.5
通讯作者：
R. Wallace
中科院分区：
文献类型：
--
作者：
P. Begovac;R. Wallace
海龙（Syngnathusscovelli）卵巢中卵母细胞的发育具有时间和空间模式。结合[3 H]胸苷摄取和细胞培养的细胞学观察被用来作为标准，以确定在这个物种的卵母细胞发育的六个阶段。第一阶段-卵囊藻，发现于杰佛逊山脊内。阶段II-初级生长的卵母细胞，细分为（1）减数分裂早期前期I的染色质-核仁卵母细胞，也发现于germinal ridge内，和（2）核仁周卵母细胞，具有多个核仁，位于定形卵泡内（Balbiani卵黄体是后者卵母细胞的主要细胞质成分）。第三阶段-卵母细胞以皮质泡、卵黄被膜和脂质的出现来区分。第四阶段-卵黄发生卵母细胞，积累卵黄蛋白，并拥有初级，过渡和成熟的卵黄球。阶段V-成熟卵母细胞，能够在体内进行最终减数分裂成熟，并在体外对类固醇17 α-羟基-20 β-二氢孕酮做出反应。第六阶段-排卵的成熟卵子，这是目前在卵巢腔，并能够受精。这些研究提供了一个分期系列的卵子发生和卵母细胞发育的尖嘴鱼，并与这些阶段不寻常的卵巢在这种动物。
Oocyte development occurs in a temporal and spatial pattern in the ovary of the pipefish, Syngnathus scovelli. Cytological observations combined with [3H]thymidine uptake and cell culture were used as criteria to identify six stages of oocyte development in this species. Stage I—Oogonia, found within the germinal ridge. Stage II—Oocytes in primary growth, subdivided into (1) chromatin‐nucleolus oocytes in early prophase I of meiosis, also found within the germinal ridge, and (2) perinucleolar oocytes, which possess multiple nucleoli and are within definitive follicles (the Balbiani vitelline body is the main cytoplasmic component in these latter oocytes). Stage III—Oocytes distinguished by the appearance of cortical alveoli, the vitelline envelope, and lipid. Stage IV—Vitellogenic oocytes, which accumulate yolk proteins and possess primary, transitional, and mature yolk spheres. Stage V—Maturational oocytes, which are competent to undergo final meiotic maturation in vivo and in response to the steroid 17 α‐hydroxy‐20β‐dihydroprogesterone in vitro. Stage VI—Ovulated mature eggs, which are present in the ovary lumen and are capable of being fertilized. These studies provide a staging series for oogenesis and oocyte development in the pipefish and relate these stages to the unusual ovary in this animal.
研究卵母细胞成熟的新形态学方法。
影响因子：
3.6
作者：
Albertini,DF
通讯作者：
Albertini,DF