Amine modification of nonporous silica nanoparticles reduces inflammatory response following intratracheal instillation in murine lungs.
Amine modification of nonporous silica nanoparticles reduces inflammatory response following intratracheal instillation in murine lungs.
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DOI：
10.1016/j.toxlet.2015.11.006
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发表时间：
2016-01-22
影响因子：
3.5
通讯作者：
Salem AK
中科院分区：
文献类型：
--
作者：
Morris AS;Adamcakova-Dodd A;Lehman SE;Wongrakpanich A;Thorne PS;Larsen SC;Salem AK
Amorphous silica nanoparticles (NPs) possess unique material properties that make them ideal for many different applications. However, the impact of these materials on human and environmental health needs to be established. We investigated nonporous silica NPs both bare and modified with amine functional groups (3-aminopropyltriethoxysilane (APTES)) in order to evaluate the effect of surface chemistry on biocompatibility. In vitro data showed there to be little to no cytotoxicity in a human lung cancer epithelial cell line (A549) for neither bare silica NPs nor amine-functionalized NPs using doses based on both mass concentration (below 200 μg/mL) and exposed total surface area (below 14 m2/L). To assess lung inflammation, C57/B16 mice were administered bare and amine-functionalized silica NPs via intra-tracheal instillation. Two doses (0.1 and 0.5 mg NPs/mouse) were tested using the in vivo model. At the higher dose used, bare silica NPs elicited a significantly higher inflammatory response, as evidence by increased neutrophils and total protein in bronchoalveolar (BAL) fluid compared to amine-functionalized NPs. From this study, we conclude that functionalization of nonporous silica NPs with APTES molecules reduces murine lung inflammation and improves the overall biocompatibility of the nanomaterial.
