Design of a synthetic yeast genome
Design of a synthetic yeast genome
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DOI：
10.1126/science.aaf4557
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发表时间：
2017-03-10
期刊：
影响因子：
56.9
通讯作者：
Bader, Joel S.
中科院分区：
文献类型：
--
作者：
Richardson, Sarah M.;Mitchell, Leslie A.;Bader, Joel S.
我们描述了一个合成的真核基因组，Sc2.0，高度修饰的酿酒酵母基因组的大小减少了近8%，与1.1兆碱基的合成基因组删除，插入或改变的完整设计。Sc2.0染色体设计是通过BioStudio实现的，BioStudio是一个为真核基因组设计开发的开源框架，它协调从核苷酸到基因组规模的设计修改，并实施版本控制以系统地跟踪编辑。为了实现完整的Sc2.0基因组合成，由Sc2.0联盟团队在世界各地构建的单个合成染色体将通过“核内复制互交”整合成单个菌株。“像Sc2.0这样的化学合成基因组是完全可定制的，允许实验者以自下而上的设计策略提出关于染色体结构、功能和进化的棘手问题。
We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome designwas implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by " endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.