Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr
Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr
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大豆子叶组织中体细胞胚的诱导
DOI：
10.1007/bf00269295
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发表时间：
1984
影响因子：
6.2
通讯作者：
G. Lippmann
中科院分区：
文献类型：
--
作者：
B. Lippmann;G. Lippmann
描述了在大豆组织培养物中诱导体细胞胚的方法。来自未成熟胚胎的子叶被用作外植体来源。在培养基中添加生长素（2,4-D、MCPA、2,4,5-T、NAA、IAA、IBA）会导致子叶外植体上分生组织的形成。分生组织形成的程度取决于培养基中生长素的种类和浓度。使用 2,4-D 和 MCPA，胚状体起源于分生组织。胚状形成率受到作为外植体来源的胚的发育阶段和生长素浓度的影响。在含有2,4-D的培养基中添加细胞分裂素或补充高糖浓度抑制了子叶外植体上分生组织和胚状体的形成。
A method for the induction of somatic embryos in soybean tissue cultures is described. Cotyledons from immature embryos were utilized as explant source. Supplementing the culture medium with auxins (2,4-D, MCPA, 2,4,5-T, NAA, IAA, IBA) caused formation of meristematic tissue on cotyledon explants. The extent of meristematic tissue formed depended on the kind and concentration of auxin in the culture medium. With 2,4-D and MCPA, embryoids originated from meristematic tissue. Embryoid formation rates were influenced by the developmental stage of the embryos serving as explant source and auxin concentration. Addition of cytokinins to the medium containing 2,4-D or supplementing it with high sugar concentrations inhibited the formation of meristematic tissue and of embryoids on cotyledon explants.